Anti-lipopolysaccharide antibody administration mitigates ruminal lipopolysaccharide release and depression of ruminal pH during subacute ruminal acidosis challenge in Holstein bull cattle
| Autor: | Hitoshi Mizuguchi, Yumi Watanabe, Kentaro Ikuta, Shigeru Sato, Yohan Kim, Shiro Kushibiki, Tomoki Ikeda |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Lipopolysaccharides
Male Rumen animal structures Lipopolysaccharide Cattle Diseases anti-lipopolysaccharide antibody chemistry.chemical_compound Animal science Latin square Internal Medicine Animals subacute ruminal acidosis Blood urea nitrogen chemistry.chemical_classification General Veterinary biology Full Paper lipopolysaccharide Fatty acid food and beverages Hydrogen-Ion Concentration Lactic acid Diet rumen fermentation chemistry cattle biology.protein Fermentation Antibody Acidosis |
| Zdroj: | The Journal of Veterinary Medical Science |
| ISSN: | 1347-7439 |
| Popis: | The effects of anti-lipopolysaccharide (LPS) antibody on rumen fermentation and LPS activity were investigated during subacute ruminal acidosis (SARA) challenge. Eleven Holstein cattle (164 ± 14 kg) were used in a 3 × 3 Latin square design. Cattle were fed a roughage diet on days -11 to -1 (pre-challenge) and day 2 (post-challenge), and a high-grain diet on days 0 and 1 (SARA challenge). For 14 days, 0-, 2-, or 4-g of anti-LPS antibody was administered once daily through a rumen fistula. Ruminal pH was measured continuously, and rumen fluid and blood samples were collected on days -1, 0, 1, and 2. Significantly lower ruminal LPS activity on day 1 was observed in the 2- and 4-g groups than those in the 0-g group. In addition, significantly higher 1-hr mean ruminal pH on SARA challenge period (days 0 and 1) was identified in the 4-g group than in the 0-g group. However, rumen fermentation measurements (total volatile fatty acid [VFA], VFA components, NH3-N and lactic acid) and peripheral blood metabolites (glucose, free fatty acid, beta-hydroxybutyrate, total cholesterol, blood urea nitrogen, aspartate aminotransferase and gamma-glutamyl transferase) were not different among the groups during the experimental periods. Therefore, anti-LPS antibody administration mitigates LPS release and pH depression without the depression of rumen fermentation and peripheral blood metabolites during SARA challenge in Holstein cattle. |
| Databáze: | OpenAIRE |
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