Chronic exposure to uranium leads to iron accumulation in rat kidney cells
| Autor: | Marie Donnadieu-Claraz, Mathilde Bonnehorgne, B. Dhieux, Christophe Maubert, François Paquet, Marie Cheynet, Patrick Gourmelon |
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| Přispěvatelé: | Laboratoire de radiotoxicologie et radiobiologie expérimentale (LRTOX), Institut de Radioprotection et de Sûreté Nucléaire (IRSN), Laboratoire de thermodynamique et physico-chimie métallurgiques (LTPCM), Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique de Grenoble (INPG) |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Male
[SDV]Life Sciences [q-bio] tissue distribution environmental exposure oral drug administration Administration Oral Urine animal cell 010501 environmental sciences Kidney 01 natural sciences Ferric Compounds law.invention Kidney Tubules Proximal Rats Sprague-Dawley iron law dose response homeostasis kidney cell rat animal Water Pollutants Radioactive Cells Cultured 0303 health sciences Radiation electron energy loss spectroscopy Cultured Sprague Dawley rat Chemistry drug effect article trace element Proximal Environmental exposure scanning electron microscope Uranium urine 3. Good health Dose–response relationship medicine.anatomical_structure bioaccumulation Kidney Tubules priority journal ferric oxide Administration microscopy Drug granular cell inorganic chemicals Oral Water Pollutants Radioactive iron oxide Metabolic Clearance Rate Cells animal experiment Biophysics chemistry.chemical_element complex mixtures water pollutant Nephrotoxicity animal tissue kidney proximal tubule Dose-Response Relationship 03 medical and health sciences medicine Animals Radiology Nuclear Medicine and imaging controlled study 030304 developmental biology 0105 earth and related environmental sciences cell culture nonhuman Dose-Response Relationship Drug concentration (parameters) Rattus animal model Radiochemistry drinking water technology industry and agriculture Rats ferric ion X irradiation cytology Sprague-Dawley Electron microscope metabolism Homeostasis |
| Zdroj: | Radiation Research Radiation Research, 2007, 167 (4), pp.454-464. ⟨10.1667/RR0545.1⟩ |
| DOI: | 10.1667/RR0545.1⟩ |
| Popis: | After it is incorporated into the body, uranium accumulates in bone and kidney and is a nephrotoxin. Although acute or short-term uranium exposures are well documented, there is a lack of information about the effects of chronic exposure to low levels of uranium on both occupationally exposed people and the general public. The objective of this study was to identify the distribution and chemical form of uranium in kidneys of rats chronically exposed to uranium in drinking water (40 mg uranium liter-1). Rats were killed humanely 6, 9, 12 and 18 months after the beginning of exposure. Kidneys were dissected out and prepared for optical and electron microscope analysis and energy dispersive X-ray (XEDS) or electron energy loss spectrometry (EELS). Microscopic analysis showed that proximal tubule cells from contaminated rats had increased numbers of vesicles containing dense granular inclusions. These inclusions were composed of clusters of small granules and increased in number with the exposure duration. Using XEDS and EELS, these characteristic granules were identified as iron oxides. Uranium was found to be present as a trace element but was never associated with the iron granules. These results suggested that the mechanisms of iron homeostasis in kidney could be affected by chronic uranium exposure. © 2007 by Radiation Research Society. |
| Databáze: | OpenAIRE |
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