Biocompatibility of nanostructured boron doped diamond for the attachment and proliferation of human neural stem cells
| Autor: | Clément Hébert, A. C. Taylor, Robert Edgington, Barbora Vagaska, Philippe Bergonzo, Patrizia Ferretti, Richard B. Jackman |
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| Přispěvatelé: | London Centre for Nanotechnology, University College of London [London] (UCL), Cardiac Unit, Institute of Child Health (UCL), Laboratoire Capteurs Diamant (LCD-LIST), Département Métrologie Instrumentation & Information (DM2I), Laboratoire d'Intégration des Systèmes et des Technologies (LIST), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Laboratoire d'Intégration des Systèmes et des Technologies (LIST), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, European Project: 280433,EC:FP7:NMP,FP7-NMP-2011-SMALL-5,NEUROCARE(2012), Laboratoire d'Intégration des Systèmes et des Technologies (LIST (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Laboratoire d'Intégration des Systèmes et des Technologies (LIST (CEA)) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
NANOCRYSTALLINE DIAMOND
Cell Culture Techniques Biocompatible Materials 02 engineering and technology 01 natural sciences law.invention DOPAMINE Neural Stem Cells law FILM ELECTRODES education.field_of_study 021001 nanoscience & nanotechnology DIFFERENTIATION 0210 nano-technology inorganic chemicals Materials science Biocompatibility Population Biomedical Engineering CULTURES chemistry.chemical_element Nanotechnology MICROELECTRODE ARRAYS Carbon nanotube engineering.material 010402 general chemistry Cellular and Molecular Neuroscience biocompatibility THIN-FILMS diamond stem cells Humans [CHIM.COOR]Chemical Sciences/Coordination chemistry [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics education Boron [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials Embryonic Stem Cells Cell Proliferation Dopant STABILITY Doping Diamond 0104 chemical sciences Nanostructures chemistry DNA SENSORS engineering IMPLANTATION Electrochemical window |
| Zdroj: | Journal of Neural Engineering Journal of Neural Engineering, IOP Publishing, 2015, 12 (6), pp.066016. ⟨10.1088/1741-2560/12/6/066016⟩ Journal of Neural Engineering, 2015, 12 (6), pp.066016. ⟨10.1088/1741-2560/12/6/066016⟩ |
| ISSN: | 1741-2560 1741-2552 |
| DOI: | 10.1088/1741-2560/12/6/066016⟩ |
| Popis: | International audience; Objective. We quantitatively investigate the biocompatibility of chemical vapour deposited (CVD) nanocrystalline diamond (NCD) after the inclusion of boron, with and without nanostructuring. The nanostructuring method involves a novel approach of growing NCD over carbon nanotubes (CNTs) that act as a 3D scaffold. This nanostructuring of BNCD leads to a material with increased capacitance, and this along with wide electrochemical window makes BNCD an ideal material for neural interface applications, and thus it is essential that their biocompatibility is investigated. Approach. Biocompatibility was assessed by observing the interaction of human neural stem cells (hNSCs) with a variety of NCD substrates including undoped ones, and NCD doped with boron, which are both planar, and nanostructured. hNSCs were chosen due to their sensitivity, and various methods including cell population and confluency were used to quantify biocompatibility. Main results. Boron inclusion into NCD film was shown to have no observable effect on hNSC attachment, proliferation and viability. Furthermore, the biocompatibility of nanostructured boron-doped NCD is increased upon nanostructuring, potentially due to the increased surface area. Significance. Diamond is an attractive material for supporting the attachment and development of cells as it can show exceptional biocompatibility. When boron is used as a dopant within diamond it becomes a p-type semiconductor, and at high concentrations the diamond becomes quasi-metallic, offering the prospect of a direct electrical device-cell interfacing system. |
| Databáze: | OpenAIRE |
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