Intrapatient Comparison of an Intermittent and a Continous Flow Cell Separator for the Collection of Progenitor and Stem Cells from the Blood
| Autor: | Fabrizio Beretta, Sabine van den Bosch, Michele Ghielmini, Franco Cavalli, Damiano Castelli |
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| Rok vydání: | 1998 |
| Předmět: |
Adult
Male Time Factors medicine.medical_treatment Cell Separation Peripheral blood mononuclear cell Blood cell medicine Humans Platelet Leukapheresis Progenitor cell Clonogenic assay Chemotherapy business.industry Hematology General Medicine Middle Aged Hematopoietic Stem Cells Blood Cell Count medicine.anatomical_structure Immunology Female Stem cell business Biomedical engineering |
| Zdroj: | Vox Sanguinis. 75:149-153 |
| ISSN: | 1423-0410 0042-9007 |
| DOI: | 10.1046/j.1423-0410.1998.7520149.x |
| Popis: | Background and Objectives: Continuous-flow and intermittent-flow blood cell separators (CFCS and IFCS) are both used to collect stem cells from the blood to rescue patients undergoing myeloablative treatment for cancer. Materials and Methods: We designed a study to compare the collection efficiency of the two systems. The continous-flow Cobe Spectra and the intermittent-flow Haemonetics MCS-3P were used to collect cells on consecutive days from 9 patients mobilised with G-CSF with or without chemotherapy. Blood obtained before leukapheresis and the leukapheresis product were analysed for their content of red and white cells, platelets, CD34-positive cells, GM-CFC, CFC-E, and BFU-E. An extraction ratio was calculated. Results: We found that the CFCS extracted about 4 times more mononuclear cells per unit time, 3 times more CD34-positive, and 4 times more clonogenic cells than the IFCS. The subject acceptability of the two systems was similar. Conclusion: The CFCS is a more efficient system for stem cell collection. IFCS requires a longer harvesting time for the same result. |
| Databáze: | OpenAIRE |
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