Pharmacological study on the mixed CCK8/DA meso-nucleus accumbens pathway: Evidence for the existence of storage sites containing the two transmitters
| Autor: | J. M. Studler, G. Blanc, Jacques Glowinski, M. Reibaud, Gérard Tramu, Jean-Pol Tassin |
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| Rok vydání: | 1984 |
| Předmět: |
Male
medicine.medical_specialty Reserpine Apomorphine Dopamine Radioimmunoassay Methyltyrosines Neuropeptide Nucleus accumbens Nucleus Accumbens Sincalide Mesencephalon Internal medicine Neural Pathways medicine Animals Molecular Biology Chemistry General Neuroscience Ventral striatum Rats Inbred Strains Corpus Striatum Rats alpha-Methyltyrosine Endocrinology medicine.anatomical_structure Monoamine neurotransmitter Mechanism of action Septal Nuclei Alpha-Methyltyrosine Neurology (clinical) medicine.symptom Developmental Biology medicine.drug |
| Zdroj: | Brain Research. 298:91-97 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/0006-8993(84)91149-1 |
| Popis: | The mixed CCK8/DA meso-nucleus accumbens pathway was used as a model to study the effects of some pharmacological treatments on the two coexisting transmitters. Reserpine (7 mg/kg i.p.), which depletes monoamine vesicles, induced as early as 1 h following its injection a selective decrease (36%) of CCK8 levels in the posterior part of the nucleus accumbens, an area innervated by the mixed CCK8/DA projection. In contrast, this treatment was without effect on CCK8 levels in the anterior nucleus accumbens and the ventral striatum, two areas which contain distinct CCK8 and DA innervations. Apomorphine (5 mg/kg i.p.), which is known to inhibit the firing rate of DA cells, did not block the reserpine- induced decrease in CCK8 levels suggesting that reserpine is acting on CCK8 storage. This mechanism of action was further substantiated by results obtained with alpha-methyl-p-tyrosine (alpha-MpT, 200 mg/kg i.p.) since no change in CCK8 levels was observed 4 h after this treatment. However, a selective decrease (35%) in CCK8 levels was found in the posterior part of the nucleus accumbens 20 h after two successive alpha-MpT injections. This suggested that long-term interruption of DA transmission resulted in an activation of CCK8/DA cells leading to a release of CCK8. The partial effect of reserpine on total CCK8 stores in CCK8/DA fibers suggests that the peptide is distributed in two types of storage compartments, one of them being sensitive to reserpine and possibly corresponding to mixed CCK8/DA vesicles. |
| Databáze: | OpenAIRE |
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