Enhanced Suppression of Immune Cells In Vitro by MSC Overexpressing FasL
| Autor: | Anca Violeta Gafencu, Ana-Maria Vacaru, Madalina Dumitrescu, Andrei Mircea Vacaru, Radu Ionita, Maya Simionescu, Ioana Madalina Fenyo |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Fas Ligand Protein
Apoptosis Biology medicine.disease_cause Lymphocyte Activation immunomodulation Catalysis Fas ligand Article Autoimmunity Inorganic Chemistry lcsh:Chemistry Mice Immune system In vivo Mice Inbred NOD Splenocyte medicine Animals Physical and Theoretical Chemistry Molecular Biology lcsh:QH301-705.5 Spectroscopy Cells Cultured Cell Proliferation immunosuppression Organic Chemistry Mesenchymal stem cell autoimmunity Cell Differentiation Mesenchymal Stem Cells General Medicine In vitro FasL Computer Science Applications lcsh:Biology (General) lcsh:QD1-999 Cancer research Female mesenchymal stromal cells Spleen |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 1 International Journal of Molecular Sciences, Vol 22, Iss 348, p 348 (2021) |
| ISSN: | 1422-0067 |
| Popis: | Mesenchymal stromal cells (MSC) display several mechanisms of action that may be harnessed for therapeutic purposes. One of their most attractive features is their immunomodulatory activity that has been extensively characterized both in vitro and in vivo. While this activity has proven to be very efficient, it is transient. We aimed to enhance it by transforming MSC to overexpress a first apoptosis signal (Fas) ligand (FasL). In this study, our goal was to induce FasL overexpression through adenoviral transduction in MSC to improve their immunomodulatory activity. We characterized the impact of FasL overexpression on the morphology, proliferation, viability, phenotype, multilineage differentiation potential and immunomodulation of MSC. Moreover, we determined their suppressive properties in mixed reactions with A20 cells, as well as with stimulated splenocytes. Our findings demonstrate that FasL-overexpressing MSC exhibit improved immunosuppressive properties, while maintaining their MSC-characteristic features. In conclusion, we establish, in a proof-of-concept set-up, that FasL-overexpressing MSC represent good candidates for therapeutic intervention targeted at autoimmune disorders. |
| Databáze: | OpenAIRE |
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