Flip-Flop of Phospholipids in Vesicles: Kinetic Analysis with Time-Resolved Small-Angle Neutron Scattering
| Autor: | Tetsurou Handa, Minoru Nakano, Masakazu Fukuda, Takuto Azuma, Naoya Matsuzaki, Hitoshi Endo, Kazuhisa Sekine, Takayuki Kudo |
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| Rok vydání: | 2009 |
| Předmět: |
Time Factors
Lipid Bilayers Neutron diffraction Neutron scattering chemistry.chemical_compound Scattering Small Angle Materials Chemistry Physical and Theoretical Chemistry POPC Phospholipids Unilamellar Liposomes Chemistry Phospholipase D Scattering Hydrolysis Vesicle Cell Membrane technology industry and agriculture Small-angle neutron scattering Surfaces Coatings and Films Kinetics Neutron Diffraction Crystallography Cholesterol Membrane lipids (amino acids peptides and proteins) |
| Zdroj: | The Journal of Physical Chemistry B. 113:6745-6748 |
| ISSN: | 1520-5207 1520-6106 |
| DOI: | 10.1021/jp900913w |
| Popis: | We applied a time-resolved small-angle neutron scattering technique to vesicle systems to determine interparticle transfer and flip-flop of phospholipids. Measurements were performed for large unilamellar vesicles, consisting of dimyristoylphosphatidylcholine (DMPC), 1-palmitoyl-2-oleoylphosphatidylcholine (POPC), or 1-palmitoyl-2-oleoylphosphatidic acid (POPA), which differ either in their acyl chains or headgroup. POPC, which is analogous to naturally occurring phosphatidylcholines, exhibited no transbilayer transfer and very slow interbilayer migration. POPC on the inner leaflet of vesicles did not flop even when phospholipase D converted all POPC molecules on the outer leaflet into POPA, which was shown to exhibit fast flip-flop. From these results, together with the observation that the flip-flop of DMPC was entirely inhibited in the presence of cholesterol, it is deduced that the flip-flop of phosphatidylcholines does not take place spontaneously in cellular plasma membranes rich in cholesterol and that it requires enzymatic activities of energy-dependent and/or -independent flippases/floppases. |
| Databáze: | OpenAIRE |
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