Low oxygen levels as a trigger for enhancement of respiratory metabolism in Saccharomyces cerevisiae
| Autor: | Mervi Toivari, Merja Penttilä, Marilyn G. Wiebe, Juha-Pekka Pitkänen, Laura Ruohonen, Eija Rintala |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Proteome
Transcription Genetic lcsh:QH426-470 Mitochondrial translation lcsh:Biotechnology Saccharomyces cerevisiae Citric Acid Cycle chemistry.chemical_element 7. Clean energy Oxygen Transcriptome 03 medical and health sciences Gene Expression Regulation Fungal lcsh:TP248.13-248.65 Genetics Cluster Analysis Anaerobiosis RNA Processing Post-Transcriptional Promoter Regions Genetic 030304 developmental biology 0303 health sciences biology 030306 microbiology Gene Expression Profiling Metabolism biology.organism_classification Lipid Metabolism Aerobiosis Mitochondria Citric acid cycle lcsh:Genetics Biochemistry chemistry Anaerobic exercise Research Article Biotechnology |
| Zdroj: | BMC Genomics, Vol 10, Iss 1, p 461 (2009) Rintala, E, Toivari, M, Pitkänen, J P, Wiebe, M G, Ruohonen, L & Penttilä, M 2009, ' Low oxygen levels as a trigger for enhancement of respiratory metabolism in Saccharomyces cerevisiae ', BMC Genomics, vol. 10, 1471 . https://doi.org/10.1186/1471-2164-10-461 BMC Genomics |
| ISSN: | 1471-2164 |
| Popis: | Background The industrially important yeast Saccharomyces cerevisiae is able to grow both in the presence and absence of oxygen. However, the regulation of its metabolism in conditions of intermediate oxygen availability is not well characterised. We assessed the effect of oxygen provision on the transcriptome and proteome of S. cerevisiae in glucose-limited chemostat cultivations in anaerobic and aerobic conditions, and with three intermediate (0.5, 1.0 and 2.8% oxygen) levels of oxygen in the feed gas. Results The main differences in the transcriptome were observed in the comparison of fully aerobic, intermediate oxygen and anaerobic conditions, while the transcriptome was generally unchanged in conditions receiving different intermediate levels (0.5, 1.0 or 2.8% O2) of oxygen in the feed gas. Comparison of the transcriptome and proteome data suggested post-transcriptional regulation was important, especially in 0.5% oxygen. In the conditions of intermediate oxygen, the genes encoding enzymes of the respiratory pathway were more highly expressed than in either aerobic or anaerobic conditions. A similar trend was also seen in the proteome and in enzyme activities of the TCA cycle. Further, genes encoding proteins of the mitochondrial translation machinery were present at higher levels in all oxygen-limited and anaerobic conditions, compared to fully aerobic conditions. Conclusion Global upregulation of genes encoding components of the respiratory pathway under conditions of intermediate oxygen suggested a regulatory mechanism to control these genes as a response to the need of more efficient energy production. Further, cells grown in three different intermediate oxygen levels were highly similar at the level of transcription, while they differed at the proteome level, suggesting post-transcriptional mechanisms leading to distinct physiological modes of respiro-fermentative metabolism. |
| Databáze: | OpenAIRE |
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