Development of a computer-based quantification method for immunohistochemically-stained tissues and its application to study mast cells in equine wound healing (proof of concept)
| Autor: | H. Richard, Sheila Laverty, Christine L. Theoret, Valérie Dubuc, Monique Doré |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Pathology
medicine.medical_specialty 040301 veterinary sciences Wound healing Inflammation Cell Count Horse Mast cell 0403 veterinary science Mast (sailing) 03 medical and health sciences Keloid Quantification medicine Image Processing Computer-Assisted Animals Horses Mast Cells 030304 developmental biology Skin 0303 health sciences lcsh:Veterinary medicine General Veterinary business.industry Methodology Article Significant difference Computer based 04 agricultural and veterinary sciences General Medicine medicine.disease Immunohistochemistry ImageJ 3. Good health medicine.anatomical_structure Granulation Tissue lcsh:SF600-1100 medicine.symptom business Software |
| Zdroj: | BMC Veterinary Research, Vol 16, Iss 1, Pp 1-8 (2020) BMC Veterinary Research |
| Popis: | Background : There is a growing interest in the scientific community to use computer-based software programs for the quantification of cells during physiological and pathophysiological processes. Drawbacks of computer-based methods currently used to quantify immunohistochemical staining are the complexity of use, expense of software and overly-simplified descriptions of protocol thereby limiting reproducibility. The precise role of mast cells in equine cutaneous wound healing is unknown. Given the contribution of mast cells to the chronic inflammation observed in human keloid, a pathology similar to exuberant granulation tissue (EGT) in horses, mast cells might be present in high numbers in equine limb wounds predisposed to EGT. The main goal of this study was to develop a reliable and reproducible quantification method for immunostained tissues using a computer software that is widely available, at no cost, to the scientific community. A secondary goal was to conduct a proof of concept using the newly-established method to quantify mast cells during wound healing at different anatomical sites (body and limb) in horses to see if a different pattern is observed in limb wounds , which are predisposed to EGT. Results : A good intraclass correlation coefficient (ICC, 0.67 p < 0.05) was found between the computer-based ImageJ method and manual counting. An excellent intra-operator ICC of 0.90 (p < 0.01) was found for the ImageJ quantification method while a good interoperator ICC of 0.69 (p < 0.01) was measured. No significant difference was observed between the variation of the ImageJ and that of the manual counting method. Mast cells were localized below the epidermis, around cutaneous appendages and blood vessels. Mast cell numbers did not differ significantly in relation to anatomical location or time of healing. Conclusions : The computer-based quantification method developed is reliable, reproducible, available, cost-free and could be used to study different physiological and pathological processes using immunohistochemistry. |
| Databáze: | OpenAIRE |
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