Chromatographic Separation and Identification of Some Peptides in Partial Hydrolysates of Gelatin

Autor:	F. Charlotte Green, Walter A. Schroeder, Lewis Honnen
Rok vydání:	1953
Předmět:	Multidisciplinary Chromatography food.ingredient Resolution (mass spectrometry) Chemical structure Sequence (biology) Gelatin Hydrolysate Chemistry chemistry.chemical_compound Chromatographic separation food chemistry Dinitrophenyl Ion-exchange resin
Zdroj:	Proceedings of the National Academy of Sciences. 39:23-30
ISSN:	1091-6490 0027-8424
DOI:	10.1073/pnas.39.1.23
Popis:	Recently we have been engaged in a study of the chemical structure of collagen and gelatin with the object of determining the sequence of the amino acid residues in the polypeptide chains of these proteins. In the course of this study we have made considerable progress in the chromatographic analysis of complex mixtures of peptides and we have isolated and identified several simple peptides which occur in partial hydrolysates of gelatin. The initial separation of the mixture into zones of one or more peptides has been made on a column of ion exchange resin; further separation of the peptides in each zone has been achieved by chromatographing in the form of dinitrophenyl (DNP) peptides on columns of silicic acid-Celite. It is to be hoped that the particular combination of chromatographic methods which has been successfully used in the present study will be helpful in the resolution of the complex mixtures which result from the partial hydrolysis of other proteins.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::33d9d4c3a0a4420d5cf684a4e1ac1b5c https://doi.org/10.1073/pnas.39.1.23 Zobrazit plný text záznamu