Natural Killer Cell Receptor+T-Lymphocytes in Normal andHelicobacter pylori-Infected Human Gastric Mucosa
| Autor: | Anthony P. Moran, Carol M. Gately, Joan O'Keeffe, Yvonne O'Donoghue, F. M. Stevens, Syed A. Zulquernain |
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| Rok vydání: | 2008 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Adolescent Population Biology Helicobacter Infections Natural killer cell Immune system T-Lymphocyte Subsets Gastric mucosa medicine Humans Receptor education Aged Lamina propria education.field_of_study Lymphokine-activated killer cell Helicobacter pylori Gastroenterology General Medicine Middle Aged Flow Cytometry Natural killer T cell CD56 Antigen Infectious Diseases medicine.anatomical_structure Gastric Mucosa Immunology Receptors Natural Killer Cell Female NK Cell Lectin-Like Receptor Subfamily D NK Cell Lectin-Like Receptor Subfamily B |
| Zdroj: | Helicobacter. 13:500-505 |
| ISSN: | 1523-5378 1083-4389 |
| Popis: | Helicobacter pylori infection is associated with development of chronic inflammation and infiltration of immune cells into the gastric mucosa. As unconventional T-lymphocytes expressing natural killer cell receptors are considered to play central roles in the immune response against infection, a study investigating their frequencies in normal and H. pylori-infected gastric mucosa was undertaken.Flow cytometry was used to quantify T-cells expressing the natural killer cell markers CD161, CD56, and CD94 in freshly isolated lymphocytes from the epithelial and lamina propria layers of gastric mucosa. Thirteen H. pylori-positive and 24 H. pylori-negative individuals were studied.CD94(+) T-cells were the most abundant (up to 40%) natural killer receptor-positive T-cell population in epithelial and lamina propria layers of H. pylori-negative gastric mucosa. CD161(+) T-cells accounted for about one-third of all T-cells in both compartments, but the lowest proportion were of CD56(+) T-cells. Compared with H. pylori-negative mucosa, in H. pylori-infected mucosa the numbers of CD161(+) T-cells were significantly greater (p = .04) in the epithelium, whereas the numbers of CD56(+) T-cells were lower (p = .01) in the lamina propria. A minor population (2%) of T-cells in both mucosal layers of H. pylori-negative subjects were natural killer T-cells, and whose proportions were not significantly different (p.05) to those in H. pylori-infected individuals.The predominance, heterogeneity, and distribution of natural killer cell receptor-positive T-cells at different locations within the gastric mucosa reflects a potential functional role during H. pylori infection and warrants further investigation. |
| Databáze: | OpenAIRE |
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