Bacterial biofilm is associated with higher levels of regulatory T cells in unaffected hidradenitis suppurativa skin

Autor:	Gregor B.E. Jemec, Jens O Eriksen, Rune Kjærsgaard Andersen, Klaus Kallenbach, Hans Christian Ring
Rok vydání:	2019
Předmět:	CD4-Positive T-Lymphocytes 0301 basic medicine Pathology medicine.medical_specialty Biopsy Cell number Interleukin-1beta Dermatology CD8-Positive T-Lymphocytes T-Lymphocytes Regulatory Biochemistry 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine medicine Humans Hidradenitis suppurativa IL-2 receptor Molecular Biology Skin Bacteria business.industry Interleukin-17 Interleukin-2 Receptor alpha Subunit Biofilm FOXP3 Forkhead Transcription Factors biochemical phenomena metabolism and nutrition medicine.disease Hidradenitis Suppurativa Staining 030104 developmental biology Biofilms Case-Control Studies business Hair Follicle Dysbiosis CD8
Zdroj:	Experimental Dermatology. 28:312-316
ISSN:	0906-6705
DOI:	10.1111/exd.13885
Popis:	Background The role of bacterial biofilm in hidradenitis suppurativa (HS) is highly debated. Less biofilm is found in clinically unaffected axillary perilesional skin of HS patients compared with healthy controls. Objective To study the correlation between biofilm and the phenotypical characterization of the preclinical inflammatory infiltrate. Materials and methods An exploratory comparative study of punch biopsies from unaffected axillary HS skin compared to similarly biopsies from healthy controls underwent standard staining procedures for CD4, CD8, CD25, FoxP3 and IL17. Standard-sized inflammatory histological hotspots were identified manually. Slides were scanned into Leica Biosystems' Digital Image Hub. Number of stained cells per slide and hotspot was found using an algorithm. Results 12.5% of HS had biofilm compared to 85% of controls. For full slides, HS patients had more CD4+ cells than controls; HS patients with biofilm had higher CD4+ cell number than controls with or without biofilm and HS patients without biofilm. For hotspots, HS patients with biofilm had higher number of CD4+FoxP3+ cells than HS patients without biofilm and controls with biofilm. Conclusion The association between biofilm and the number of regulatory T cells in HS patients supports the concept of dysbiosis as a factor in the preclinical HS lesions.
Databáze:	OpenAIRE
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