Hydrogen peroxide stimulates uropathogenic Escherichia coli strains to cellulose production
| Autor: | Wioletta Adamus-Białek, Katrin Janik, Tara L. Vollmerhausen |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
030106 microbiology chemistry.chemical_element medicine.disease_cause Microbiology Peroxide Oxygen 03 medical and health sciences chemistry.chemical_compound Immune system medicine Humans Uropathogenic Escherichia coli Cellulose Hydrogen peroxide Escherichia coli Escherichia coli Infections biology Biofilm Hydrogen Peroxide biochemical phenomena metabolism and nutrition biology.organism_classification 030104 developmental biology Infectious Diseases chemistry Biofilms Urinary Tract Infections Reactive Oxygen Species Bacteria |
| Zdroj: | Microbial pathogenesis. 126 |
| ISSN: | 1096-1208 |
| Popis: | Reactive oxygen intermediates, such as hydrogen peroxide, are toxic molecules produced by immune cells in response to bacterial invasion into the host. Bacteria try to protect themselves against the immune system through specific properties such as biofilm formation. This phenomenon occurs also during urinary tract infections. Cellulose is an important factor of Escherichia coli biofilm and contributes to building a protective shield around bacterial cells upon the host immune response. In this study, we aimed to analyze the effect of hydrogen peroxide on the production of this biofilm component. To achieve this goal, 25 clinical E. coli strains isolated from patients with urinary tract infections were used. These bacterial strains were characterized based on their growth characteristics, their ability to form biofilm and their capacity to produce cellulose upon exposure to sub-lethal concentrations of hydrogen peroxide growth, and the biofilm formation of these strains was analyzed. Our results revealed that the analyzed uropathogenic E. coli strains slightly, but significantly, reduced growth and biofilm production upon hydrogen peroxide treatment. However, when separating these strains regarding their ability to produce cellulose, we found that general biofilm production was reduced but cellulose expression was induced upon peroxide treatment. This finding contributes to a better understanding of how bacterial biofilm formation is triggered and provides interesting insights into how uropathogenic E. coli protect themselves in an inhospitable environment. |
| Databáze: | OpenAIRE |
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