Intranasal Delivery of Genistein-Loaded Nanoparticles as a Potential Preventive System against Neurodegenerative Disorders
Autor: | Nina Senes, Silvia Fancello, Antonella Obinu, Paolo Giunchedi, Elena Piera Porcu, Elisabetta Gavini, Rossana Migheli, Giovanna Rassu, Grazia Galleri |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Pharmaceutical Science Nanoparticle Genistein lcsh:RS1-441 02 engineering and technology Pharmacology Article Chitosan genistein lcsh:Pharmacy and materia medica 03 medical and health sciences chemistry.chemical_compound Sodium hexametaphosphate neurodegenerative disease In vivo intranasal delivery sodium hexametaphosphate Cytotoxicity Chemistry 021001 nanoscience & nanotechnology Bioavailability 030104 developmental biology Nasal administration nanoparticles nose-to-brain chitosan 0210 nano-technology |
Zdroj: | Pharmaceutics, Vol 11, Iss 1, p 8 (2018) Pharmaceutics Volume 11 Issue 1 |
ISSN: | 1999-4923 |
Popis: | Genistein has been reported to have antioxidant and neuroprotective activity. Despite encouraging in vitro and in vivo results, several disadvantages such as poor water solubility, rapid metabolism, and low oral bioavailability limit the clinical application of genistein. The aim of this study was to design and characterize genistein-loaded chitosan nanoparticles for intranasal drug delivery, prepared by the ionic gelation technique by using sodium hexametaphosphate. Nanoparticles were characterized in vitro and their cytotoxicity was tested on PC12 cells. Genistein-loaded nanoparticles were prepared, and sodium hexametaphosphate was used as a valid alternative to well-known cross-linkers. Nanoparticle characteristics as well as their physical stability were affected by formulation composition and manufacturing. Small (mean diameters of 200&ndash 300 nm) and homogeneous nanoparticles were obtained and were able to improve genistein penetration through the nasal mucosa as compared to pure genistein. Nanoparticle dispersions showed a pH consistent with the nasal fluid and preserved PC12 cell vitality. |
Databáze: | OpenAIRE |
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