Plant sterols and casein-derived tripeptides attenuate blood pressure increase in spontaneously hypertensive rats

Autor: Riitta Korpela, Aino Siltari, Anne S. Kivimäki, Anu M. Turpeinen, Pauliina I. Ehlers, Heikki Vapaatalo
Rok vydání: 2012
Předmět:
Male
medicine.medical_specialty
Nitric Oxide Synthase Type III
Endothelium
030309 nutrition & dietetics
Endocrinology
Diabetes and Metabolism
Anti-Inflammatory Agents
Down-Regulation
Blood Pressure
Enzyme-Linked Immunosorbent Assay
Tripeptide
Peptidyl-Dipeptidase A
030204 cardiovascular system & hematology
Real-Time Polymerase Chain Reaction
Nitric oxide
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Endocrinology
Rats
Inbred SHR
Internal medicine
medicine
Animals
Endothelial dysfunction
Antihypertensive Agents
0303 health sciences
Nutrition and Dietetics
Chemistry
Caseins
Phytosterols
Drug Synergism
medicine.disease
Angiotensin II
Rats
3. Good health
P-Selectin
Blood pressure
medicine.anatomical_structure
Cyclooxygenase 2
Hypertension
Sodium nitroprusside
Asymmetric dimethylarginine
medicine.drug
Zdroj: Nutrition Research. 32:292-300
ISSN: 0271-5317
DOI: 10.1016/j.nutres.2012.03.004
Popis: In this study, we investigated the synergistic effects of plant sterols (PS) and casein-derived tripeptides on arterial tone and blood pressure in experimental hypertension. We hypothesized that PS and tripeptides could have positive, synergistic effects on the development of hypertension and endothelial dysfunction in young spontaneously hypertensive rats (SHR). Six-week-old male SHR were divided into 3 groups to receive milk products containing PS, or PS with tripeptides, or a control containing no active components for 8 weeks. Systolic blood pressure (SBP) was measured weekly, and vascular reactivity measurements with isolated mesenteric arteries were performed at the end of the study. Biochemical measurements for several parameters were performed by enzyme-linked immunosorbent assay using plasma samples. Levels of angiotensin-converting enzyme 1, cyclooxygenase-2, endothelial nitric oxide synthase, and P-selectin messenger RNA expressions were determined from aortic tissue by real-time polymerase chain reaction. The study showed that long-term treatment with PS + tripeptides attenuated the development of hypertension in SHR (SBP, 187 ± 5 mm Hg vs 169 ± 4 mm Hg in control group; P < .01). Plant sterols alone did not affect SBP significantly. Endothelial dysfunction was observed in all SHR; however, treatment with PS resulted in poorer endothelium-dependent and nitric oxide–mediated relaxation compared with other groups. Aortic cyclooxygenase-2 and P-selectin were significantly down-regulated in PS and PS + tripeptides groups when compared with the control group. The expression of endothelial nitric oxide synthase was significantly lower in PS than in PS + tripeptides group. In conclusion, long-term treatment with PS has a slight but not significant antihypertensive effect. Plant sterols do not provide any beneficial effects on endothelial function in hypertensive rats; however, treatment with both PS and tripeptides showed mild anti-inflammatory effects.
Databáze: OpenAIRE
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