Effects of meta-tetrahydroxyphenylchlorin photodynamic therapy on isogenic colorectal cancer SW480 and SW620 cells with different metastatic potentials
| Autor: | Yuhua Li, Gulinur Abdulrehman, Ling Kang, Kaiyue Xv |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Programmed cell death Light Cell Survival medicine.medical_treatment Photodynamic therapy Apoptosis Dermatology Flow cytometry 03 medical and health sciences 0302 clinical medicine Cell Movement Cell Line Tumor medicine Humans MTT assay Neoplasm Metastasis Cytotoxicity Tumor Stem Cell Assay Photosensitizing Agents medicine.diagnostic_test Cell growth Chemistry 030104 developmental biology Mesoporphyrins Photochemotherapy Cell culture 030220 oncology & carcinogenesis Cancer research Surgery Colorectal Neoplasms Subcellular Fractions |
| Zdroj: | Lasers in medical science. 33(7) |
| ISSN: | 1435-604X |
| Popis: | The aim of this study is to investigate the antitumor effects and possible mechanisms of meta-tetrahydroxyphenylchlorin-mediated photodynamic therapy (m-THPC-PDT) on human primary (SW480) and metastatic (SW620) colon cancer cell lines. SW480 and SW620 cells were incubated with various concentrations of m-THPC, followed by photodynamic irradiation. Subcellular localization of m-THPC in cells was observed with confocal laser scanning microscopy (CLSM). Photocytotoxicity of m-THPC in the two cells was investigated by using MTT assay. The flow cytometry was employed to detect the cell apoptosis. The migration and long-term recovery ability were determined by scratch test and colony formation assay respectively. CLSM showed that m-THPC was mainly distributed within the endoplasmic reticulum (ER) and lysosome of SW480 cells and within the lysosome and mitochondria of SW620 cells. m-THPC-PDT induced a dose-dependent and light energy-dependent cytotoxicity in SW480 and SW620 cells. Apoptosis rate was approximately 65 and 25% in SW480 and SW620 respectively when the concentration of m-THPC increased to 11.76 μM. However, the rate of necrotic cells had no significant changes in two cell lines. The colony formation and migration ability of the two cell lines were decreased with m-THPC-PDT treatment in a dose-dependent manner. PDT with m-THPC not only could effectively inhibit cell proliferation and decrease migration ability and colony formation ability, but also could effectively kill SW480 and SW620 cells in a dose-dependent manner in vitro. These results suggest that m-THPC is a promising sensitizer that warrants further development and extensive studies towards clinical use of colorectal cancer. |
| Databáze: | OpenAIRE |
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