Detection of Epstein-Barr virus-specific memory CD4+T cells using a peptide-based cultured enzyme-linked immunospot assay
| Autor: | Paola Zelini, Sandra A. Calarota, Fausto Baldanti, Lorenzo Minoli, Antonella Chiesa, Giuditta Comolli |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male Enzyme-Linked Immunospot Assay Epstein-Barr Virus Infections Herpesvirus 4 Human Immunology Immunodominance Biology medicine.disease_cause Virus Immunocompromised Host Interferon-gamma Immune system Antigen Interferon hemic and lymphatic diseases medicine Humans Immunology and Allergy Antigens Viral Cells Cultured Aged ELISPOT Reproducibility of Results Original Articles Middle Aged Epstein–Barr virus Virology BZLF1 Heart Transplantation Female Immunocompetence Immunologic Memory Biomarkers Immunosuppressive Agents Interferon-gamma Release Tests Lung Transplantation medicine.drug |
| Zdroj: | Immunology. 139:533-544 |
| ISSN: | 0019-2805 |
| DOI: | 10.1111/imm.12106 |
| Popis: | Approaches to evaluate T-cell responses to Epstein–Barr virus (EBV) include enzyme-linked immunospot (ELISPOT), which quantifies cells capable of immediate interferon-γ secretion upon antigen stimulation. However, evaluation of expandable EBV-specific memory T cells in an ELISPOT format has not been described previously. We quantified EBV-specific T-cell precursors with high proliferative capacity by using a peptide-based cultured interferon-γ ELISPOT assay. Standard and cultured ELISPOT responses to overlapping peptide pools (15-mers overlapping by 11 amino acids) covering the lytic (BZLF1 and BMRF1) and latent (EBNA1, EBNA3a, EBNA3b, EBNA3c, LMP1 and LMP2) EBV proteins were evaluated in 20 healthy subjects with remote EBV infection and, for comparison, in four solid organ transplant recipients. Cultured ELISPOT responses to both lytic and latent EBV antigens were significantly higher than standard ELISPOT responses. The distribution of EBV-specific T-cell responses detected in healthy virus carriers showed more consistent cultured ELISPOT responses compared with standard ELISPOT responses. T-cell responses quantified by cultured ELISPOT were mainly mediated by CD4+ T cells and a marked pattern of immunodominance to latent-phase antigens (EBNA1 > EBNA3 family antigens > LMP2 > LMP1) was shown. Both the magnitude and distribution of EBV-specific T-cell responses were altered in solid organ transplant recipients; in particular, cultured ELISPOT responses were almost undetectable in a lung-transplanted patient with EBV-associated diseases. Analysis of T-cell responses to EBV by ELISPOT assays might provide new insights into the pathogenesis of EBV-related diseases and serve as new tools in the monitoring of EBV infection in immunocompromised patients. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |