Classification of archaic rice grains excavated at the Mojiaoshan site within the Liangzhu site complex reveals an Indica and Japonica chloroplast complex
| Autor: | Masaaki Kanehara, Tetsuro Udatsu, Bin Liu, Shinji Kubota, Ningyuan Wang, Cailin Wang, Nobuhiko Kamijo, Chunfang Zhao, Ryuji Ishikawa, Katsunori Tanaka, Minghui Chen, Minako Kanehara, Hiroyuki Tasaki, Shin-ichi Nakamura |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Nuclear gene lcsh:TX341-641 01 natural sciences Genome Japonica 03 medical and health sciences Genetic resources Botany Plastid Domestication Research data Diversity lcsh:TP368-456 biology food and beverages Agriculture Archaic DNA biology.organism_classification Chloroplast lcsh:Food processing and manufacture 030104 developmental biology Archaeology Rice Mojiaoshan lcsh:Nutrition. Foods and food supply 010606 plant biology & botany |
| Zdroj: | Food Production, Processing and Nutrition, Vol 2, Iss 1, Pp 1-12 (2020) |
| ISSN: | 2661-8974 |
| DOI: | 10.1186/s43014-020-00028-8 |
| Popis: | Abstract To understand rice types that were utilized during postdomestication and in the modern age and the potential of genetic research in aged rice materials, archaeogenetic analysis was conducted for two populations of archaic rice grains from the Mojiaoshan site during the Liangzhu Period in China (2940 to 2840 BC). Sequencing after the PCR amplification of three regions of the chloroplast genome and one region of the nuclear genome showed recovery rates that were comparable to those in previous studies except for one chloroplast genome region, suggesting that the materials used in this work were appropriate for recovering genetic information related to domestication traits by using advanced technology. Classification after sequencing in these regions proved the existence of Japonica and Indica chloroplasts in archaic grains from the west trench, which were subsequently classified into eight plastid groups (type I–VIII), and indicated that these rice grains derived from different maternal lineages were stored together in storage houses at the Mojiaohsan site. Among these plastid groups, type V exhibited the same sequences as two modern Indica accessions that are utilized in basic studies and rice breeding. It was inferred that part of the chloroplast genome of archaic rice has been preserved in modern genetic resources in these two modern Indica accessions, and the results indicated that rice related to their maternal ancestor was present at the Mojiaoshan site during the Liangzhu Period in China. The usefulness of archaeogenetic analysis can be demonstrated by our research data as well as previous studies, providing encouragement for the possibility that archaeogenetic analysis can be applied to older rice materials that were utilized in the rice-domesticated period. Graphical abstract |
| Databáze: | OpenAIRE |
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