Effect of PML and PML-RAR on the transactivation properties and subcellular distribution of steroid hormone receptors
| Autor: | A Guiochon-Mantel, J F Savouret, F Quignon, K Delabre, E Milgrom, H De The |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Transcriptional Activation
Transcription Genetic Receptors Retinoic Acid Recombinant Fusion Proteins medicine.medical_treatment Molecular Sequence Data Retinoic acid CHO Cells Promyelocytic Leukemia Protein Transfection Cell Line Promyelocytic leukemia protein Transactivation chemistry.chemical_compound Endocrinology Tretinoin Cricetinae Progesterone receptor medicine Animals Humans Receptor Molecular Biology Base Sequence biology Tumor Suppressor Proteins Nuclear Proteins General Medicine Fusion protein Neoplasm Proteins Cell biology Steroid hormone chemistry biology.protein Cancer research Receptors Progesterone HeLa Cells Transcription Factors medicine.drug |
| Zdroj: | Molecular Endocrinology. 9:1791-1803 |
| ISSN: | 1944-9917 0888-8809 |
| Popis: | PML (promyelocytic leukemia) is a protein involved in the t (15;17) translocation of promyelocytic leukemia and is mainly localized in nuclear bodies. Here we show that PML exerts a very powerful enhancing activity (up to 20-fold) on the transactivating properties of the progesterone receptor (PR) and has a similar effect on several other steroid hormone receptors. There is probably a direct or indirect interaction between PR and PML, because when the latter was expressed at high concentrations it shifted PR into the nuclear bodies. The use of deletion mutants showed that both activation functions (AF1 and AF2) of PR as well as the coiled coil and His-Cys-rich domains of PML were required for transcriptional enhancement. The fusion protein PML-RAR which is not localized in nuclear bodies, also enhanced the transactivating activity of PR, but this effect was totally suppressed by the administration of retinoic acid. PML, which is ubiquitously expressed, may thus be involved in the transactivation properties of steroid hormone receptors. This mechanism may also play a role in the oncogenic properties of PML-RAR and in their suppression by retinoic acid. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|