Prime-boost vaccination with recombinant protein and adenovirus-vector expressing Plasmodium vivax circumsporozoite protein (CSP) partially protects mice against Pb/Pv sporozoite challenge
| Autor: | Irene S. Soares, Alba Marina Gimenez, Katia Sanches Françoso, Chiara Andolina, Luciana Lima, Mauricio M. Rodrigues, François Nosten, Tarsila Mendes de Camargo, Elisângela Oliveira de Freitas, Laurent Rénia, karina de Almeida Caramico, Ruth S. Nussenzweig, Arturo Reyes-Sandoval, Hildegund C.J. Ertl, Victor Nussenzweig, Oscar Bruna-Romero |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Serotype Plasmodium vivax Genetic Vectors Antibody Affinity Protozoan Proteins lcsh:Medicine Antibodies Protozoan Parasitemia medicine.disease_cause Article Viral vector Adenoviridae 03 medical and health sciences Mice Immunogenicity Vaccine parasitic diseases Malaria Vaccines medicine Malaria Vivax Animals Amino Acid Sequence VACINAÇÃO lcsh:Science Multidisciplinary biology Immunogenicity lcsh:R Plasmodium falciparum biology.organism_classification medicine.disease Virology Recombinant Proteins 3. Good health Circumsporozoite protein Disease Models Animal 030104 developmental biology Immunoglobulin G lcsh:Q Female Immunization |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Scientific Reports Scientific Reports, Vol 8, Iss 1, Pp 1-14 (2018) |
| Popis: | Vaccine development against Plasmodium vivax malaria lags behind that for Plasmodium falciparum. To narrow this gap, we administered recombinant antigens based on P. vivax circumsporozoite protein (CSP) to mice. We expressed in Pichia pastoris two chimeric proteins by merging the three central repeat regions of different CSP alleles (VK210, VK247, and P. vivax-like). The first construct (yPvCSP-AllFL) contained the fused repeat regions flanked by N- and C-terminal regions. The second construct (yPvCSP-AllCT) contained the fused repeat regions and the C-terminal domain, plus RI region. Mice were vaccinated with three doses of yPvCSP in adjuvants Poly (I:C) or Montanide ISA720. We also used replication-defective adenovirus vectors expressing CSP of human serotype 5 (AdHu5) and chimpanzee serotype 68 (AdC68) for priming mice which were subsequently boosted twice with yPvCSP proteins in Poly (I:C) adjuvant. Regardless of the regime used, immunized mice generated high IgG titres specific to all CSP alleles. After challenge with P. berghei ANKA transgenic parasites expressing Pb/PvVK210 or Pb/PvVK247 sporozoites, significant time delays for parasitemia were observed in all vaccinated mice. These vaccine formulations should be clinically tried for their potential as protective universal vaccine against P. vivax malaria. |
| Databáze: | OpenAIRE |
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