Gating a Single Cell: A Label-Free and Real-Time Measurement Method for Cellular Progression
| Autor: | Peter K.N. Yu, Shishir Venkatesh, Vaithinathan Karthikeyan, Chi Chung Yeung, Saw Lin Oo, Abdul Mojeed Ilyas, Xianfeng Chen, Vellaisamy A. L. Roy, Eva Yi Kong, Clement Manohar Arava |
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| Rok vydání: | 2020 |
| Předmět: |
In situ
Time Factors Cells Cell Apoptosis Gating 010402 general chemistry 01 natural sciences Mass Spectrometry Analytical Chemistry HeLa medicine Tumor Cells Cultured Humans Electrodes Ions Measurement method biology Chemistry potassium 010401 analytical chemistry Cancer medicine.disease biology.organism_classification 0104 chemical sciences Membrane medicine.anatomical_structure Cancer cell Biophysics Irradiation Single-Cell Analysis HeLa Cells |
| Zdroj: | Oo, S L, Venkatesh, S, Ilyas, A M, Karthikeyan, V, Arava, C M, Kong, E Y, Yeung, C C, Chen, X, Yu, P K N & Roy, V A L 2020, ' Gating a Single Cell : A Label-Free and Real-Time Measurement Method for Cellular Progression ', Analytical Chemistry, vol. 92, no. 2, pp. 1738-1745 . https://doi.org/10.1021/acs.analchem.9b03136 |
| ISSN: | 1520-6882 |
| DOI: | 10.1021/acs.analchem.9b03136 |
| Popis: | There is an ever-growing need for more advanced methods to study the response of cancer cells to new therapies. To determine cancer cells' response from a cell-mortality perspective to various cancer therapies, we report a label-free and real time method to monitor the in situ response of individual HeLa cells using a single cell gated transistor (SCGT). As a cell undergoes apoptotic cell death, it experiences changes in morphology and ion concentrations. This change is well in line with the threshold voltage of the SCGT, which has been verified by correlating the data with the cell morphologies by scanning electron microscopy and the ion-concentration analysis by inductively-coupled plasma mass spectrometry (ICPMS). This SCGT could replace patch clamps to study single cell activity via direct measurement in real time. Importantly, this SCGT can be used to study the electrical response of a single cell to stimuli that leaves the membrane intact. |
| Databáze: | OpenAIRE |
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