Immunohistochemical mapping of pro-opiomelanocortin- and pro-dynorphin-derived peptides in the alpaca (Lama pacos) diencephalon
| Autor: | Zaida Díaz-Cabiale, Rafael Coveñas, L.A. Aguilar, Manuel Lisardo Sánchez, B. Manso, J.A. Narváez, L.E. Medina |
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| Rok vydání: | 2014 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Pro-Opiomelanocortin Alpha-neo-endorphin Thalamus Hypothalamus Dynorphin Biology Alpha-melanocyte-stimulating hormone Cellular and Molecular Neuroscience chemistry.chemical_compound Diencephalon Arcuate nucleus Internal medicine medicine Animals Protein Precursors Opioid peptide Brain Chemistry Neurons Adrenocorticotropin hormone Enkephalins purl.org/pe-repo/ocde/ford#3.01.04 [https] Immunohistochemistry alpha-Melanocyte-stimulating hormone Beta-endorphin Endocrinology Leucine-enkephalin nervous system chemistry beta-Endorphin Camelids New World hormones hormone substitutes and hormone antagonists |
| Zdroj: | Journal of Chemical Neuroanatomy. :36-50 |
| ISSN: | 0891-0618 |
| Popis: | Using an indirect immunoperoxidase technique, we studied the distribution of cell bodies and fibres containing non-opioid peptides (adrenocorticotropin hormone (ACTH), alpha-melanocyte-stimulating hormone) and opioid peptides (beta-endorphin (1-27), alpha-neo-endorphin, leucine-enkephalin) in the alpaca diencephalon. No immunoreactive cell bodies containing ACTH were found. Perikarya containing the other four peptides were observed exclusively in the hypothalamus and their distribution was restricted. Perikarya containing alpha-melanocyte-stimulating hormone or alpha-neo-endorphin showed a more widespread distribution than those containing leucine-enkephalin or beta-endorphin (1-27). Cell bodies containing pro-opiomelanocortin-derived peptides were observed in the arcuate nucleus, anterior and lateral hypothalamic areas and in the ventromedial and supraoptic hypothalamic nuclei, whereas perikarya containing alpha-neo-endorphin (a pro-dynorphin-derived peptide) were found in the arcuate nucleus, dorsal and lateral hypothalamic areas, and in the paraventricular, ventromedial and supraoptic hypothalamic nuclei. Immunoreactive cell bodies containing leucine-enkephalin were found in the lateral hypothalamic area and in the paraventricular hypothalamic nucleus. Immunoreactive fibres expressing pro-opiomelanocortin-derived peptides were more numerous than those expressing pro-dynorphin-derived peptides. A close anatomical relationship was observed: in all the diencephalic nuclei in which beta-endorphin (1-27)-immunoreactive fibres were found, fibres containing alpha-melanocyte-stimulating hormone or alpha-neo-endorphin were also observed. Fibres containing beta-endorphin (1-27), alpha-melanocyte-stimulating hormone or alpha-neo-endorphin were widely distributed throughout the diencephalon, but fibres containing ACTH or leucine-enkephalin showed a moderate distribution. The distribution of the five peptides studied here is also compared with that reported previously in other mammalian species. The widespread distribution observed indicates that both the pro-dynorphin and the pro-opiomelanocortin systems are involved in multiple physiological actions (e.g., food intake, thermoregulation, neuroendocrine and reproductive mechanisms) in the alpaca diencephalon. |
| Databáze: | OpenAIRE |
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