Click by Click Microporous Annealed Particle (MAP) Scaffolds
| Autor: | Tatiana Segura, Weixian Xi, Nicole J. Darling, Elias Sideris, Cassie Pong, S. Thomas Carmichael, Alexa R. Anderson |
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| Rok vydání: | 2019 |
| Předmět: |
Scaffold
Materials science Biocompatibility Medical Biotechnology Biomedical Engineering Pharmaceutical Science norbornene Biocompatible Materials 02 engineering and technology 010402 general chemistry Regenerative Medicine 01 natural sciences Article Biomaterials Medicinal and Biomolecular Chemistry Tetrazine chemistry.chemical_compound Hyaluronic acid Humans Hyaluronic Acid Porosity Tissue Engineering Tissue Scaffolds stroke models porous scaffolds technology industry and agriculture Hydrogels Dynamic mechanical analysis Microporous material Fibroblasts 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Chemical engineering Click chemistry tetrazine microbeads 0210 nano-technology |
| Zdroj: | Adv Healthc Mater Advanced healthcare materials, vol 9, iss 10 |
| Popis: | Macroporous scaffolds are being increasingly used in regenerative medicine and tissue repair. While our recently developed microporous annealed particle (MAP) scaffolds have overcome issues with injectability and in situ hydrogel formation, limitations with respect to tunability to be able to manipulate hydrogel strength and rigidity for broad applications still exist. To address these key issues, here we synthesized hydrogel microparticles (HMPs) of hyaluronic acid (HA) using the thiol-norbornene click reaction and then subsequently annealed HMPs into a porous scaffold using the tetrazine-norbornene click reaction. This assembly method allowed for straightforward tuning of bulk scaffold rigidity by varying the tetrazine to norbornene ratio, with increasing tetrazine resulting in increasing scaffold storage modulus, Young’s modulus, and maximum stress. These changes were independent of void fraction. Further incorporation of human dermal fibroblasts (HDFs) throughout the porous scaffold revealed the biocompatibility of this annealing strategy as well as differences in proliferation and cell-occupied volume. Finally, injection of porous HA-Tet MAP scaffolds into an ischemic stroke model showed this chemistry is biocompatible in vivo with reduced levels of inflammation and astrogliosis as previously demonstrated for other crosslinking chemistries. |
| Databáze: | OpenAIRE |
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