Characterization of a holin (HolNU3â1) in methicillin-resistantStaphylococcus aureushost
| Autor: | Toshinobu Horii, Miya Kobayashi, Yasuhiro Suzuki |
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| Rok vydání: | 2002 |
| Předmět: |
Microbiology (medical)
Staphylococcus aureus Molecular Sequence Data Immunology Lysin Biology medicine.disease_cause Microbiology Virus Evolution Molecular Bacteriophage Bacterial Proteins medicine Humans Immunology and Allergy Bacteriophages Amino Acid Sequence Cloning Molecular Gene Sequence Homology Amino Acid N-Acetylmuramoyl-L-alanine Amidase Sequence Analysis DNA General Medicine Staphylococcal Infections biology.organism_classification Methicillin-resistant Staphylococcus aureus Infectious Diseases Holin Methicillin Resistance Bacteria |
| Zdroj: | FEMS Immunology & Medical Microbiology. 34:307-310 |
| ISSN: | 1574-695X 0928-8244 |
| Popis: | The gene encoding holin protein HolNU3-1 from a clinical isolate of methicillin-resistant Staphylococcus aureus (MRSA) NU3-1 was cloned and expressed in S. aureus RN4220. HolNU3-1 encoded by the holNU3-1 gene, which is located upstream of the deleted endolysin gene, was functional. Expression of the holNU3-1 gene induced a decrease in culture turbidity and formation of translucent (empty ghost) cells in S. aureus. We found heterogeneity of the holin genes and diversity of the two-component lysis system, which consists of holin and endolysin, in MRSA hosts. We suggest that this diversity is important in the identification of the evolution of clinical isolates of S. aureus. |
| Databáze: | OpenAIRE |
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