Editor’s Highlight: Effects of Intraperitoneal Injection of SnS2 Flowers on Mouse Testicle
| Autor: | Lu Yuan, Qingzhao Li, Disi Bai, Peijun Shen, Ping Wu, Yanjie Xiong, Jun-Jian Zhao, Liyuan Bai |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Surface Properties medicine.medical_treatment Intraperitoneal injection Gene Expression Nitric Oxide Synthase Type II Apoptosis Spleen Sulfides 010501 environmental sciences Testicle Toxicology 01 natural sciences Andrology 03 medical and health sciences chemistry.chemical_compound Testis medicine Animals Tissue Distribution Particle Size 0105 earth and related environmental sciences Mice Inbred ICR Dose-Response Relationship Drug Sperm Count Tin Compounds Malondialdehyde Sperm Nanostructures 030104 developmental biology medicine.anatomical_structure chemistry Cyclooxygenase 2 Toxicity Cytokines Reproductive toxicity Injections Intraperitoneal |
| Zdroj: | Toxicological Sciences. 161:388-400 |
| ISSN: | 1096-0929 1096-6080 |
| DOI: | 10.1093/toxsci/kfx220 |
| Popis: | SnS2 nanoflowers (SnS2 NFs) have been widely used in photoelectric and catalytic applications. However, its explosure and reproductive toxicity is unknown. The aim of this study was to investigate the effect of exposure to 3 different sized-SnS2 flowers (dose: 38 mg/kg; size: 50, 80, and 200 nm) in testes of mice for 4 weeks by intraperitoneal injection. Though the body weight of mice treated or not with SnS2 NFs was not different, and SnS2 NFs were distributed to the organs including liver, kidney, spleen, heart, brain, and testis, more distribution SnS2 NFs (50 and 80 nm) were found in testicle tissues compared with SnS2 flowers (200 nm) in those tissues. The results of sperm count and survival analysis, histopathological evaluation, and qRT-PCR detection showed that there was moderate reproductive toxicity induced by the small-sized SnS2 NFs in testicle tissues. Furthermore, elevated malondialdehyde level and decreased superoxide dismutase activity were also observed in the SnS2 NFs (dose: 38 mg/kg; size: 50 and 80 nm) treated groups. Likewise, the qRT-PCR data indicated that SnS2 NFs can induce apoptosis and inflammation responses. Although the pro-inflammation marker of TNF-α, IL-1β, iNOS, and COX-2 at the mRNA levels were higher expression in 50 and 80 nm groups than that in control and 200 nm group, no statistical significance existed between 50 and 80 nm groups. Accordingly, the repeated-dose toxicity of SnS2 NFs in testicle tissues was also observed in a dose-dependent manner by intraperitoneal injection of SnS2 NFs (size: 50 nm; 0.38, 3.8, and 38 mg/kg) for 4 weeks, when determined by sperm count, survival rate, and qRT-PCR analysis. In addition, transmission electron microscopy showed that the ultrastructural abnormalities formed by the small-sized SnS2 NFs in testes were more severe than those formed by the large-sized SnS2 in testes. Taken together, these findings implied that the SnS2 NFs activated inflammation responses that signified apoptosis in murine testes. This study provided useful information for risk analysis and regulation of SnS2 NFs by administration agencies. |
| Databáze: | OpenAIRE |
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