Repeated toluene exposure leads to neuroadaptation in dopamine release mechanisms within the nucleus accumbens core
| Autor: | Sean P. Callan, Scott E. Bowen, Aaron K. Apawu, Tiffany A. Mathews |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Microdialysis Dopamine Fast-scan cyclic voltammetry Nucleus accumbens Pharmacology Toxicology Nucleus Accumbens Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Neurochemical medicine Animals Neurotransmitter Dopaminergic 030104 developmental biology chemistry 030220 oncology & carcinogenesis Solvents Autoreceptor Locomotion Toluene medicine.drug |
| Zdroj: | Toxicology and Applied Pharmacology. 408:115260 |
| ISSN: | 0041-008X |
| Popis: | Background Intentionally inhaling volatile organic solvent like toluene for its intoxicating effects continues to be a public health concern. While repeated abuse of toluene has deleterious behavioral and health effects, little is known about the actions of toluene on the dopaminergic neurotransmitter system within the central nervous system. Method The present study employed complementary neurochemical techniques of slice fast-scan cyclic voltammetry (FSCV) and in vivo microdialysis, to assess dopamine (DA) dynamics immediately after repeated exposure to 2000- or 4000-ppm toluene. DA D3 autoreceptor functionality, measured by FSCV with pharmacological manipulations and brain tissue content analysis with high performance liquid chromatography, were also used to account for the changes in the DA dynamics. Results Toluene-exposed mice had decreased stimulated DA release only in the nucleus accumbens core immediately after seven days of repeated exposure. DA uptake was decreased in the core only after 2000-ppm exposure. The differences in stimulated DA release were not attributed to alterations in intraneuronal DA levels as measured by tissue content analysis. Basal extracellular DA levels were not significantly different between the air- and toluene-treated mice. However, following an additional toluene exposure, mice had elevated extracellular DA levels in the nucleus accumbens during recovery. This potentiation in extracellular accumbal DA levels was further heightened following potassium stimulation. The accumbal DA D3 autoreceptor function did not appear to play a role as a potential mediator for these differences. Conclusion Our FSCV and microdialysis results suggest a neuroadaptation in DA release mechanics within the nucleus accumbens, but the exact neuronal mechanism of toluene's impact remains elusive. |
| Databáze: | OpenAIRE |
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