Single‐cell analyses reveal functional classification of dendritic cells and their potential roles in inflammatory disease
| Autor: | Qian Shi, Na Li, Yuan-Xiu Chen, Ai-Hong Yao, Ji-Ting Liu, Xian-Bin Su, Yue Han, Shan-Shan Li, Zong-Lai Jiang, Fei Zhuang, Qing-Ping Yao, Ying-Xin Qi |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
CCR2 T-Lymphocytes Receptors CCR1 CCR4 chemical and pharmacologic phenomena Spleen Biology Biochemistry Flow cytometry Mice 03 medical and health sciences Chemokine receptor 0302 clinical medicine Genetics medicine Animals Antigen-presenting cell Molecular Biology Inflammation medicine.diagnostic_test Scavenger Receptors Class A TLR9 hemic and immune systems Dendritic Cells Flow Cytometry Cell biology Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Single-Cell Analysis 030217 neurology & neurosurgery CD80 Biotechnology |
| Zdroj: | The FASEB Journal. 33:3784-3794 |
| ISSN: | 1530-6860 0892-6638 |
| DOI: | 10.1096/fj.201801489r |
| Popis: | Dendritic cells (DCs) have crucial roles in immune-related diseases. However, it is difficult to explore DCs because of their rareness and heterogeneity. Although previous studies had been performed to detect the phenotypic characteristics of DC populations, the functional diversity has been ignored. Using a combination of flow cytometry, single-cell quantitative PCR, and bioinformatic analysis, we depicted the DC panorama with not only phenotypic but also functional markers. Functional classification of DCs in mouse lymphoid tissue (spleen) and nonlymphoid tissue (liver) was performed. The results revealed that expression of macrophage scavenger receptor 1 ( MSR1) and C-C motif chemokine receptors ( CCR) 1, CCR2, and CCR4 were elevated in liver DCs, suggesting increased lipid uptake and migration abilities. The enriched expression of costimulatory molecule CD80, TLR9, and TLR adaptor MYD88 in spleen DCs indicated a more-mature phenotype, enhanced pathogen recognition, and T-cell stimulation abilities. Furthermore, we compared DCs in the atherosclerotic mouse models with healthy controls. In addition to the quantitative increase in DCs in the liver and spleen of the apolipoprotein E-knockout ( ApoE-/-) mice, the functional expression patterns of the DCs also changed at the single-cell level. These results promote our understanding of the participation of DCs in inflammatory diseases and have potential applications in DC clinical assessment.-Shi, Q., Zhuang, F., Liu, J.-T., Li, N., Chen, Y.-X., Su, X.-B., Yao, A.-H., Yao, Q.-P., Han, Y., Li, S.-S., Qi, Y.-X., Jiang, Z.-L. Single-cell analyses reveal functional classification of dendritic cells and their potential roles in inflammatory disease. |
| Databáze: | OpenAIRE |
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