Astragaloside IV and cycloastragenol stimulate the phosphorylation of extracellular signal-regulated protein kinase in multiple cell types
| Autor: | Calvin B. Harley, Lisa Y. Yung, Nancy Y. Ip, Haihong Pang, Yueqing Hu, Maurice K.C. Ho, Wing See Lam, Fanny C.F. Ip, Allison C. Chin, Yung Hou Wong |
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| Rok vydání: | 2011 |
| Předmět: |
MAPK/ERK pathway
Telomerase Sapogenins Pharmaceutical Science Analytical Chemistry Cell Line chemistry.chemical_compound Drug Discovery Humans Cycloastragenol Epidermal growth factor receptor Breast Phosphorylation Protein kinase A Extracellular Signal-Regulated MAP Kinases Lung Pharmacology biology Dose-Response Relationship Drug Plant Extracts Organic Chemistry Brain Endothelial Cells Astragalus Plant Fibroblasts Saponins Triterpenes Cell biology ErbB Receptors src-Family Kinases Complementary and alternative medicine chemistry Biochemistry Mitogen-activated protein kinase biology.protein Molecular Medicine Proto-oncogene tyrosine-protein kinase Src |
| Zdroj: | Planta medica. 78(2) |
| ISSN: | 1439-0221 |
| Popis: | Two Chinese herb-derived small molecule telomerase activators, astragaloside IV (AG-IV) and cycloastragenol (CAG), have recently been shown to improve the proliferative response of CD8+ T lymphocytes from HIV-infected patients by upregulating telomerase activity. Here, we examined the signaling mechanism of AG-IV and CAG. Telomerase activity in human embryonic kidney HEK293 fibroblasts was increased upon treatment with increasing concentrations of AG-IV or CAG. Both compounds induced the phosphorylation of extracellular signal-regulated protein kinase (ERK) in a time- and dose-dependent manner in HEK293 cells and HEK-neo keratinocytes. AG-IV and CAG also stimulated ERK phosphorylation in other cell lines of lung, brain, mammary, endothelial, and hematopoietic origins. Use of selective inhibitors and dominant negative mutants revealed the involvement of c-Src, MEK (ERK kinase), and epidermal growth factor receptor in CAG-induced ERK phosphorylation. Our data indicate that AG-IV and CAG may exert their cellular effects through the activation of the Src/MEK/ERK pathway. |
| Databáze: | OpenAIRE |
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