Gene expression of islet cell antigen p69 in human, mouse, and rat
| Autor: | Wolfram Karges, Massimo Pietropaolo, Hans-Michael Dosch, Cameron A Ackerley |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Male
endocrine system medicine.medical_specialty DNA Complementary Endocrinology Diabetes and Metabolism Molecular Sequence Data Gene Expression Mice Inbred Strains Biology Autoantigens Polymerase Chain Reaction Cell Line Islets of Langerhans Mice Fetus Western blot Internal medicine Gene expression Testis Internal Medicine medicine Animals Humans Northern blot RNA Messenger Rats Wistar Microscopy Immunoelectron Pancreas Blood–testis barrier DNA Primers geography geography.geographical_feature_category medicine.diagnostic_test Base Sequence Pancreatic islets Brain Islet Blotting Northern Immunohistochemistry Cell biology Rats Blotting Southern Kinetics Endocrinology medicine.anatomical_structure Cell culture Organ Specificity PAX4 Female |
| Zdroj: | Diabetes. 45(4) |
| ISSN: | 0012-1797 |
| Popis: | Based on the detection of specific antibodies and T-cell sensitization in patients with IDDM, islet cell antigen p69 (ICAp69) has been suggested to be a target antigen of diabetic autoimmunity. The biological function, tissue expression, and developmental kinetics of ICAp69 are largely unknown. We analyzed ICAp69 expression at the gene transcription and protein level in human and rodent tissues. By using template-calibrated quantitative reverse transcriptase polymerase chain reaction (RT-PCR), high levels of ICAp69 mRNA were found in human pancreatic islets and brain. In mouse and rat, ICAp69 gene expression peaked in islet cell lines followed by testis, islets, and brain. ICAp69 mRNA was found at low levels in other organs by RT-PCR but not by Northern blot analysis. In mice, ICAp69 transcription becomes detectable in fetal life, and fetal and adult gene expression patterns are similar. Western blot analysis of human and mouse tissues showed high expression of ICAp69 in brain, testis, pancreatic tissue, and islet cell lines. In these organs, ICAp69 immunoreactivity is predominately localized at the blood brain barrier (capillary endothelium), at the blood testis barrier (Sertoli cells and spermatids), and in pancreatic islets (β-cells). The subcellular localization of ICAp69 to endoplasmic reticulum, Golgi complex, and vesicles by immune electron microscopy suggests a role of this neuroendocrine molecule in cellular protein traffic and processing. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|