Genome Analyses of Single Human Oocytes
| Autor: | Rong Li, X. Sunney Xie, Liying Yan, Fuchou Tang, Wei Fan, Jinsen Li, Yu Hou, Jie Qiao, Ying Lian, Jin Huang, Liya Xu |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
medicine.medical_treatment Fertilization in Vitro Polar Bodies Biology Genome Polymorphism Single Nucleotide General Biochemistry Genetics and Molecular Biology Polar body Meiosis medicine Humans Genetics In vitro fertilisation Pronucleus business.industry Genome Human Biochemistry Genetics and Molecular Biology(all) MALBAC Obstetrics and Gynecology General Medicine Sequence Analysis DNA Oocyte Aneuploidy Tissue Donors medicine.anatomical_structure Blastocyst Oocytes Chromatid Female Single-Cell Analysis business |
| Zdroj: | Cell. 155(7):1492-1506 |
| ISSN: | 0092-8674 |
| DOI: | 10.1016/j.cell.2013.11.040 |
| Popis: | SummarySingle-cell genome analyses of human oocytes are important for meiosis research and preimplantation genomic screening. However, the nonuniformity of single-cell whole-genome amplification hindered its use. Here, we demonstrate genome analyses of single human oocytes using multiple annealing and looping-based amplification cycle (MALBAC)-based sequencing technology. By sequencing the triads of the first and second polar bodies (PB1 and PB2) and the oocyte pronuclei from same female egg donors, we phase the genomes of these donors with detected SNPs and determine the crossover maps of their oocytes. Our data exhibit an expected crossover interference and indicate a weak chromatid interference. Further, the genome of the oocyte pronucleus, including information regarding aneuploidy and SNPs in disease-associated alleles, can be accurately deduced from the genomes of PB1 and PB2. The MALBAC-based preimplantation genomic screening in in vitro fertilization (IVF) enables accurate and cost-effective selection of normal fertilized eggs for embryo transfer.PaperFlick |
| Databáze: | OpenAIRE |
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