Isolation of Pure Populations of Epithelial and Myoepithelial Cells from the Normal Human Mammary Gland Using Immunomagnetic Separation with Dynabeads
| Autor: | R. C. Coombes, Qiu Yan Liu, R. C. Coope, P. J. Browne, Lakjaya Buluwela, J. J. Gomm |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Cell type
DNA Complementary medicine.drug_class Molecular Sequence Data Biophysics Gene Expression Cell Separation Biology Monoclonal antibody Immunomagnetic separation Biochemistry Dynabeads Tissue culture Organoid medicine Humans Breast Molecular Biology Cells Cultured Electrophoresis Agar Gel Membrane Glycoproteins Base Sequence Immunomagnetic Separation Mucin-1 Mucins Myoepithelial cell Antibodies Monoclonal Epithelial Cells Cell Biology Molecular biology Chemically defined medium Female Neprilysin |
| Zdroj: | Analytical Biochemistry. 226:91-99 |
| ISSN: | 0003-2697 |
| Popis: | Monoclonal antibodies to epithelial membrane antigen and common acute lymphoblastic leukemia antigen were bound to second antibody-coated magnetic microspheres. These specific antibody/bead complexes were then used to directly isolate purified epithelial and myoepithelial cells from normal breast organoid cell preparations by magnetic separation. Near homogeneous cell populations were selected with yields of 10-20 × 10 6 epithelial and myoepithelial cells per organoid preparation. Repeated purification steps allowed almost complete depletion of myoepithelial cells for RNA studies. Tissue culture of separated cell populations in appropriate defined media further ensured purity of cell type and was unimpeded by Dynabead attachment. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect