Targeted oncogene activation by site-specific recombination in transgenic mice
| Autor: | Heiner Westphal, Brian Sauer, Eric J. Lee, Merja Lakso, R W Manning, S H Yu, B Mosinger, K L Mulder |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Genetically modified mouse
Antigens Polyomavirus Transforming Transgene Molecular Sequence Data Cre recombinase Mice Transgenic Regulatory Sequences Nucleic Acid Biology Cataract Mice Viral Proteins Recombinase Animals Site-specific recombination Gene Floxing Recombination Genetic Multidisciplinary Base Sequence Integrases Eye Neoplasms Oncogenes Molecular biology Oligodeoxyribonucleotides Regulatory sequence DNA Nucleotidyltransferases Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 89:6232-6236 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | An efficient and accurate method for controlled in vivo transgene modulation by site-directed recombination is described. Seven transgenic mouse founder lines were produced carrying the murine lens-specific alpha A-crystallin promoter and the simian virus 40 large tumor-antigen gene sequence, separated by a 1.3-kilobase-pair Stop sequence that contains elements preventing expression of the large tumor-antigen gene and Cre recombinase recognition sites. Progeny from two of these lines were mated with transgenic mice expressing the Cre recombinase under control of either the murine alpha A-crystallin promoter or the human cytomegalovirus promoter. All double-transgenic offspring developed lens tumors. Subsequent analysis confirmed that tumor formation resulted from large tumor-antigen activation via site-specific, Cre-mediated deletion of Stop sequences. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|