Improvement of a twice collection method of mouse oocytes by surgical operation
| Autor: | Kana Harada, Masatoshi Ooga, Sayaka Wakayama, Rei Inoue, Teruhiko Wakayama |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
Ovulation Oocyte Time Factors Offspring Midazolam medicine.medical_treatment media_common.quotation_subject Embryonic Development Oocyte Retrieval Fertilization in Vitro Reuse Biology In vitrofertilization (IVF) Intracytoplasmic sperm injection Andrology Mice 03 medical and health sciences 0302 clinical medicine medicine Animals Sperm Injections Intracytoplasmic Blastocyst 030304 developmental biology media_common 0303 health sciences 030219 obstetrics & reproductive medicine Germinal vesicle In vitro fertilisation Medetomidine Embryo Transfer Spermatozoa medicine.anatomical_structure Butorphanol Oocytes Female Original Article Animal Science and Zoology Intracytoplasmic sperm injection (ICSI) medicine.drug |
| Zdroj: | The Journal of Reproduction and Development |
| ISSN: | 1348-4400 0916-8818 |
| Popis: | Mouse oocytes are generally collected after euthanasia. However, if oocytes were collected without euthanasia, then mice could be used to collect oocytes again after recovery. This condition is especially useful for mice that are genotypically rare. In this study, we examined the reusability of mice after collecting oocytes via a surgical operation. When oocytes were collected using medetomidine/midazolam/butorphanol combination anesthesia and examined for the quality of oocytes after in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI), they could develop to full term at the same rate as controls. When oocytes were collected from those mice a second time, the average number of oocytes was reduced by nearly 1/3. However, the blastocyst and offspring rates of those oocytes after IVF or ICSI were the same as those of the control regardless of the recovery day period. Although germinal vesicle (GV) oocytes can be collected from all reused mice, the final number of offspring did not increase. Interestingly, when oocytes were collected from the front position of the ampulla, 76% of the oviducts possessed oocytes after reuse, and the average number of oocytes significantly increased to a level comparable to that of the control. Finally, we examined whether reused mice can be used as recipient females, and then healthy offspring were obtained similarly as the control recipients. In conclusion, we provide a new method to collect a sufficient number of oocytes from reused mice without concern. |
| Databáze: | OpenAIRE |
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