Novel Sensitive Immunosensor for the Selective Detection of Engrailed 2 Urinary Prostate Cancer Biomarker
| Autor: | Meriem Mokni, Amal Tlili, Ghada Attia, Sohayb Khaoulani, Chouki Zerrouki, Asma Omezzine, Ali Othmane, Ali Bouslama, Najla Fourati |
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| Rok vydání: | 2022 |
| Předmět: |
Male
History Polymers and Plastics Biomedical Engineering Biophysics Nerve Tissue Proteins Serum Albumin Human Biosensing Techniques Industrial and Manufacturing Engineering Phosphates Limit of Detection Biomarkers Tumor Electrochemistry Humans Business and International Management Early Detection of Cancer Homeodomain Proteins Immunoassay Prostate Prostatic Neoplasms Electrochemical Techniques General Medicine Prostate-Specific Antigen Immunoglobulin G Gold 4-Aminobenzoic Acid Transcription Factors Biotechnology |
| Zdroj: | SSRN Electronic Journal. |
| ISSN: | 1556-5068 |
| Popis: | Engrailed 2 (EN2) is a homeodomain-containing transcription factor expressed in prostate cancer (PCa) cell lines and is secreted into the urines. It is nowadays considered as a promising non-invasive biomarker for PCa early diagnosis. Herein, we report the design of an electrochemical immunosensor for EN2 detection. The biosensor fabrication involved a covalent immobilization of anti-EN2 antibodies onto a poly para amino benzoic acid (PABA) film electropolymerized on a gold electrode. Square wave voltammetry was investigated for EN2 detection in a phosphate buffer solution in a concentration range of 10 |
| Databáze: | OpenAIRE |
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