Development of Tg(UAS:SEC-Hsa.ANXA5-YFP,myl7:RFP); Casper(roy−/−,nacre−/−) Transparent Transgenic In Vivo Zebrafish Model to Study the Cardiomyocyte Function
| Autor: | Aaron Gopal, Vishal Arora, Surendra Kumar Rajpurohit, May Ye Mon, Nikhil Patel |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
QH301-705.5 Transgene Confocal ved/biology.organism_classification_rank.species transgenic strain Skin Pigmentation cardiomyocyte transparent skin mutant-Casper(roy−/− nacre−/−) annexin-5 Animals Genetically Modified 03 medical and health sciences 0302 clinical medicine In vivo Protocol Fluorescence microscope Animals Myocytes Cardiac fluorescent screening Annexin A5 Biology (General) Model organism Zebrafish Microphthalmia-Associated Transcription Factor Microscopy Confocal biology ved/biology Chemistry fungi General Medicine Zebrafish Proteins zebrafish biology.organism_classification Cell biology Luminescent Proteins 030104 developmental biology Models Animal in vivo confocal imaging MYL7 cellular phenotype 030217 neurology & neurosurgery Preclinical imaging |
| Zdroj: | Cells, Vol 10, Iss 1963, p 1963 (2021) Cells |
| ISSN: | 2073-4409 |
| DOI: | 10.3390/cells10081963 |
| Popis: | The zebrafish provided an excellent platform to study the genetic and molecular approach of cellular phenotype-based cardiac research. We designed a novel protocol to develop the transparent transgenic zebrafish model to study annexin-5 activity in the cardiovascular function by generating homozygous transparent skin Casper(roy−/−,nacre−/−); myl7:RFP; annexin-5:YFP transgenic zebrafish. The skin pigmentation background of any vertebrate model organism is a major obstruction for in vivo confocal imaging to study the transgenic cellular phenotype-based study. By developing Casper(roy−/−,nacre−/−); myl7; annexin-5 transparent transgenic zebrafish strain, we established time-lapse in vivo confocal microscopy to study cellular phenotype/pathologies of cardiomyocytes over time to quantify changes in cardiomyocyte morphology and function over time, comparing control and cardiac injury and cardio-oncology. Casper contributes to the study by integrating a transparent characteristic in adult zebrafish that allows for simpler transparent visualization and observation. The Casper(roy−/−,nacre−/−) transgenic progenies developed through cross-breeding with the transgenic strain of Tg(UAS:SEC-Hsa.ANXA5-YFP,myl7:RFP). Confocal and fluorescent microscopy were being used to obtain accurate, precise imaging and to determine fluorescent protein being activated. This study protocol was conducted under two sections; 1.1: Generation of homozygous Tg(UAS:SEC-Hsa.ANXA5-YFP,myl7:RFP); Casper(roy−/−,nacre−/−) zebrafish (generation F01-F06) and 1.2: Screening and sorting the transparent transgenic progeny and in vivo imaging to validate cardiac morphology through in vivo confocal imaging. We coined the newly developed strain as Tg(UAS:SEC-Hsa.ANXA5-YFP,myl7:RFP); Casper(roy−/−,nacre−/−)gmc1. Thus, the newly developed strain maintains transparency of the skin throughout the entire life of zebrafish and is capable of application of a non-invasive in vivo imaging process. These novel results provide an in vivo whole organism-based platform to design high-throughput screening and establish a new horizon for drug discovery in cardiac cell death and cardio-oncology therapeutics and treatment. |
| Databáze: | OpenAIRE |
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