Marked epithelial to mesenchymal transition in surgical margins of oral cancer-an in vitro study
| Autor: | Milos Lazarevic, Bosko Toljic, Jelena Milasin, Federica Baldan, Miroslav Dragovic, Sanja Milenkovic, Drago Jelovac, Tijana Subotički, Maja Milošević, Dijana Trišić, Zvezdana Tepavčević, Giuseppe Damante |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
surgical margins Cancer Research Cell type Cell Vimentin cell proliferation and migration Cell proliferation Epithelial to mesenchymal transition Gene expression Migration Oral cancer Surgical margins Biology 03 medical and health sciences 0302 clinical medicine medicine Epithelial–mesenchymal transition Cell growth epithelial to mesenchymal transition Cell migration Cell cycle oral cancer 030104 developmental biology medicine.anatomical_structure Oncology Cell culture 030220 oncology & carcinogenesis Cancer research biology.protein gene expression |
| Zdroj: | Oncology Letters |
| Popis: | Epithelial to mesenchymal transition (EMT) is a feature of several types of human cancer, including oral squamous cell carcinoma (OSCC). In the present study, tumor and margin cell cultures obtained from patients with OSCC were used to determine the expression patterns of certain EMT-associated markers, including vimentin, alpha -smooth muscle actin, SLUG and SNAIL. In addition, other EMT-associated features, including clonal, proliferative and migratory potential were compared between the two cell types. Cell cultures were generated from tumor and margin tissue samples from 6 patients and cultured up to the fifth passage. EMT marker expression was assessed by reverse transcription-quantitative PCR. Cell proliferation, colony formation and scratch wound healing assays were conducted to characterize the two cell types in terms of proliferation rates, clonality and motility. All of the studied markers were expressed in tumor and margin cells. Although no significant differences were noted with regard to the aforementioned markers, their expression tended to be higher in margin cultures than in tumor cultures. The expressions of the EMT markers were also higher in the fifth passage compared with those noted at the first with a few exceptions. The rates of proliferation and cell migration were decreased during passages, while the number of colonies was increased in both types of cell culture. Tumor and margin cells indicated certain similarities with regard to EMT transition characteristics. |
| Databáze: | OpenAIRE |
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