Influence of melatonin receptor 1A gene polymorphisms on seasonal reproduction in Sarda ewes with different body condition scores and ages
| Autor: | Vincenzo Carcangiu, Maria Consuelo Mura, Giovanni Cosso, Sebastiano Luridiana, Maria Luisa Diaz, Cinzia Daga, Pier Paolo Bini, Sara Bodano |
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| Rok vydání: | 2014 |
| Předmět: |
Aging
medicine.medical_specialty media_common.quotation_subject Andrology Melatonin Exon Endocrinology Food Animals Internal medicine Genotype medicine Animals Circadian rhythm media_common Sheep biology Receptor Melatonin MT1 Reproduction General Medicine biology.organism_classification Breed Melatonin receptor 1A Body Composition Female Animal Science and Zoology Sarda Seasons medicine.drug |
| Zdroj: | Animal Reproduction Science. 149:173-177 |
| ISSN: | 0378-4320 |
| Popis: | In several species, circadian changes in melatonin concentrations play a key role in the photoperiodic control of seasonality. In sheep, two silent mutations in the melatonin receptor 1A gene (MTNR1A) at positions 606 and 612 of the exon II are associated with seasonal reproduction. However, in some sheep breeds, no relationships have been found between MTNR1A polymorphisms and reproductive seasonality. This lack of relationship could be due to effects of breed, body condition, age, and/or environmental conditions. Thus, the present study was conducted with the Sarda sheep breed with the aim of documenting the effect of MTNR1A gene polymorphisms on reproductive resumption and to evaluate whether such this effect was modified by differences in body condition score (BCS) and age. Six hundred three- to six-year-old multiparous ewes with BCSs between 2.5 and 3.5 were selected. Genomic DNA was extracted and subjected to PCR to amplify the ovine exon II of the MTNR1A gene. The amplicons were subjected to digestion with the restriction enzymes RsaI and MnlI to detect the T606C and A612G polymorphisms, respectively. Ewes carrying the G/G, G/A, C/C, and C/T genotypes exhibited higher fertility rates (P |
| Databáze: | OpenAIRE |
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