Functional analysis finds differences on the muscle transcriptome of pigs fed an n-3 PUFA-enriched diet with or without antioxidant supplementations

Autor:	Roberta Davoli, Marika Vitali, Martina Zappaterra, Domenico Pietro Lo Fiego, Paolo Zambonelli, Rubina Sirri, Giulia Giannini
Přispěvatelé:	Vitali, Marika, Sirri, Rubina, Zappaterra, Martina, Zambonelli, Paolo, Giannini, Giulia, Lo Fiego, Domenico Pietro, Davoli, Roberta
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	0301 basic medicine Male Genetics and Molecular Biology (all) Antioxidant Swine medicine.medical_treatment Sus scrofa Gene Expression Organic chemistry Biochemistry Antioxidants Transcriptome Mathematical and Statistical Techniques Biochemistry Genetics and Molecular Biology (all) Agricultural and Biological Sciences (all) Flax Medicine and Health Sciences Vitamin E Food science Musculoskeletal System Flowering Plants chemistry.chemical_classification Mammals Multidisciplinary Muscles Eukaryota food and beverages 04 agricultural and veterinary sciences Vitamins Plants Physical sciences Chemistry medicine.anatomical_structure Vertebrates Medicine Female Anatomy Polyunsaturated fatty acid Research Article Meat Science Research and Analysis Methods 03 medical and health sciences Selenium Chemical compounds Lipid oxidation Fatty Acids Omega-3 Organic compounds medicine Genetics Animals Gene Regulation RNA Messenger Muscle Skeletal Nutrition Gene Expression Profiling 0402 animal and dairy science Organisms Fatty acid Skeletal muscle Polyphenols Biology and Life Sciences 040201 dairy & animal science Animal Feed Diet 030104 developmental biology chemistry Skeletal Muscles Polyphenol Dietary Supplements Amniotes Functional Analysis
Zdroj:	PLoS ONE, Vol 14, Iss 2, p e0212449 (2019) PLoS ONE
ISSN:	1932-6203
Popis:	Supplementing pig diets with n-3 polyunsaturated fatty acids (n-3 PUFA) may produce meat products with an increased n-3 fatty acid content, and the combined antioxidants addition could prevent lipid oxidation in the feed. However, to date, the effects of these bioactive compounds at the molecular level in porcine skeletal muscle are mostly unknown. This study aimed to analyse changes in the Longissimus thoracis transcriptome of 35 pigs fed three diets supplemented with: linseed (L); linseed, vitamin E and Selenium (LES) or linseed and plant-derived polyphenols (LPE). Pigs were reared from 80.8 ± 5.6 kg to 151.8 ± 9.9 kg. After slaughter, RNA-Seq was performed and 1182 differentially expressed genes (DEGs) were submitted to functional analysis. The L vs LES comparison did not show differences, while L vs LPE showed 1102 DEGs and LES vs LPE 80 DEGs. LPE compared to the other groups showed the highest number of up-regulated genes involved in preserving muscle metabolism and structure. Results enlighten that the combined supplementation of bioactive lipids (n-3 PUFA from linseed) with plant extracts as a source of polyphenols increases, compared to the only addition of linseed, the expression of genes involved in mRNA metabolic processes and transcriptional regulation, glucose uptake and, finally, in supporting muscle development and physiology. These results improve the knowledge of the biological effect of bioactive compounds in Longissimus thoracis muscle, and sustain the growing interest over their use in pig production.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::33910c9929ed18f7d2c75671b96c224b https://doaj.org/article/5246fc07ea2b4407aa71ee074a87bd12 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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