Initiation of HIV-1 reverse transcription and functional role of nucleocapsid-mediated tRNA/viral genome interactions
| Autor: | Pierre Barraud, Roland Marquet, Valérie Goldschmidt, Jean-Christophe Paillart, Dona Sleiman, Carine Tisné |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Cancer Research
Magnetic Resonance Spectroscopy viruses RNA-dependent RNA polymerase RNA Reverse Transcription Nuclear magnetic resonance spectroscopy Biology gag Gene Products Human Immunodeficiency Virus Molecular biology Genome Reverse transcriptase Infectious Diseases Transcription (biology) Virology Transfer RNA HIV-1 Nucleic Acid Conformation RNA Transfer Lys RNA Viral Primer binding site Molecular Chaperones |
| Zdroj: | Virus Research. 169:324-339 |
| ISSN: | 0168-1702 |
| Popis: | HIV-1 reverse transcription is initiated from a tRNA(Lys)(3) molecule annealed to the viral RNA at the primer binding site (PBS). The annealing of tRNA(Lys)(3) requires the opening of its three-dimensional structure and RNA rearrangements to form an efficient initiation complex recognized by the reverse transcriptase. This annealing is mediated by the nucleocapsid protein (NC). In this paper, we first review the actual knowledge about HIV-1 viral RNA and tRNA(Lys)(3) structures. Then, we summarize the studies explaining how NC chaperones the formation of the tRNA(Lys)(3)/PBS binary complex. Additional NMR data that investigated the NC interaction with tRNA(Lys)(3) D-loop are presented. Lastly, we focused on the additional interactions occurring between tRNA(Lys)(3) and the viral RNA and showed that they are dependent on HIV-1 isolates, i.e. the sequence and the structure of the viral RNA. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect