Micropropagation by tissue culture triggers differential expression of infectious endogenousBanana streak virussequences (eBSV) present in the B genome of natural and synthetic interspecific banana plantains
| Autor: | Pierre-Yves Teycheney, Michel Folliot, Yannick Lamagnère, Marie-Line Iskra-Caruana, Serge Galzi, François Xavier Cote |
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| Rok vydání: | 2010 |
| Předmět: |
Soil Science
Plant Science Virus Tissue culture Multiplication végétative Musa balbisiana Plant virus Banana streak virus Molecular Biology H20 - Maladies des plantes Génome biology food and beverages Musa Virus des végétaux Original Articles biology.organism_classification Virology Musaceae F02 - Multiplication végétative des plantes Micropropagation Culture de tissu Caulimoviridae Agronomy and Crop Science Genome Plant |
| Zdroj: | Molecular Plant Pathology |
| ISSN: | 1364-3703 1464-6722 |
| DOI: | 10.1111/j.1364-3703.2009.00583.x |
| Popis: | The genome of Musa balbisiana spp. contains several infectious endogenous sequences of Banana streak virus (eBSV). We have shown previously that in vitro micropropagation triggers the activation of infectious eBSOLV (endogenous sequences of Banana streak Obino l'Ewai virus) in the synthetic tetraploid interspecific hybrid FHIA21 (AAAB). In this work, we show that another synthetic tetraploid (AAAB) hybrid and two natural triploid (AAB) plantains are equally prone to the activation of infectious eBSOLV during tissue culture. These results are a strong indication that such activation is a general phenomenon in interspecific Musa cultivars, whether synthetic or natural. We also report the first in-depth study of the correlation between the duration of tissue culture and the level of activation of infectious eBSOLV, and show that specific and common activation patterns exist in these banana plants. We hypothesize that these patterns result from the concomitant activation of infectious eBSOLV and a decrease in the virus titre in neoformed plantlets, resulting from cell multiplication outcompeting virus replication. We provide experimental data supporting this hypothesis. No activation of infectious eBSGFV (endogenous sequences of Banana streak Goldfinger virus) by tissue culture was observed in the two natural AAB plantain cultivars studied here, whereas such activation occurred in the AAAB synthetic hybrid studied. We demonstrate that this differential activation does not result from differences in the structure of eBSGFV, as all banana genomes harbour eaBSGFV-7. |
| Databáze: | OpenAIRE |
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