Influence of clodronate and compressive force on IL-1ß-stimulated human periodontal ligament fibroblasts
| Autor: | Heiner Wehrbein, Eva Wolff, Andreas Pabst, Sarah Grimm, Ambili Mundethu, Collin Jacobs, Cornelius Jacobs, Christian Walter |
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| Rok vydání: | 2018 |
| Předmět: |
medicine.medical_specialty
Tooth Movement Techniques Periodontal Ligament Interleukin-1beta Matrix metalloproteinase Dinoprostone 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Internal medicine Gene expression medicine Periodontal fiber Humans Prostaglandin E2 General Dentistry Cells Cultured Tissue Inhibitor of Metalloproteinase-1 Chemistry 030206 dentistry Fibroblasts In vitro Biomechanical Phenomena Compressive strength Endocrinology Matrix Metalloproteinase 8 030220 oncology & carcinogenesis Tooth movement Clodronic Acid medicine.drug |
| Zdroj: | Clinical oral investigations. 24(1) |
| ISSN: | 1436-3771 |
| Popis: | The aim of this study was to investigate in vitro the effect of clodronate on interleukin-1s (IL-1s)–stimulated human periodontal ligament fibroblasts (HPdLFs) with the focus on inflammatory factors of orthodontic tooth movement with and without compressive force. HPdLFs were incubated with 5 μM clodronate and 10 ng/mL IL-1s. After 48 h, cells were exposed to 3 h of compressive force using a centrifuge. The gene expression of cyclooxygenase-2 (COX-2), interleukin-6 (IL-6), matrix metalloproteinase 8 (MMP-8), and the tissue inhibitor of MMP (TIMP-1) was analyzed using RT-PCR. Prostaglandin E2 (PGE-2), IL-6, and TIMP-1 protein syntheses were quantified via ELISA. Compressive force and IL-1s induced an overexpression of COX-2 gene expression (61.8-fold; p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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