| Autor: |
Rui Hu, Cuifang Liu, Wenlong Lu, Guanghao Wei, Dapeng Yu, Wei Li, Ping Chen, Guohong Li, Qing Zhao |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Nano letters. 22(3) |
| ISSN: |
1530-6992 |
| Popis: |
Post-translational modifications (PTMs), such as ubiquitination, are critically important in regulating genetic expressions by adjusting the nucleosome stability. A fast and label-free technology inspecting dynamic nucleosome structures can facilitate the interrogation of PTMs effects. Here we leverage the advantages of mechanically stable solid-state nanopores and detect the effect of a ubiquitinated histone on mononucleosomes at the single-molecule level. By comparing the translocation dynamics of natural and cross-linked mononucleosomes, we verified that the nucleosomal DNA unravelled from histones in natural mononucleosomes. Furthermore, we found that a turning point of voltage corresponds to the onset of nucleosome rupture. More importantly, we reveal that ubH2A stabilizes the nucleosome by shifting the turning point to a larger value and investigated the effect of ubiquitination on different histones (ubH2A and ubH2B). These findings open promising possibilities for developing a miniaturized and portable device for the fast screening of PTMs on nucleosomes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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