Isolation of 'Side Population' Progenitor Cells From Healthy Arteries of Adult Mice
| Autor: | Giuseppina Caligiuri, Julie Sainz, Dominique Urbain, Corinne Demerens, Mathilde Lemitre, Ayman Al Haj Zen, Antoine Lafont |
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| Rok vydání: | 2006 |
| Předmět: |
Pathology
medicine.medical_specialty Calponin Cell Culture Techniques Aorta Thoracic Biocompatible Materials Cell Separation Methylcellulose Mice Vasculogenesis Side population Myosin medicine ATP Binding Cassette Transporter Subfamily G Member 2 Animals Aorta Abdominal Progenitor cell Interleukin 3 biology Stem Cells Age Factors Cell Differentiation Flow Cytometry Molecular biology Mice Inbred C57BL Endothelial stem cell Drug Combinations Phenotype biology.protein ATP-Binding Cassette Transporters Female Proteoglycans Collagen Laminin Stem cell Tunica Media Cardiology and Cardiovascular Medicine Biomarkers |
| Zdroj: | Arteriosclerosis, Thrombosis, and Vascular Biology. 26:281-286 |
| ISSN: | 1524-4636 1079-5642 |
| DOI: | 10.1161/01.atv.0000197793.83391.91 |
| Popis: | Objective— Circulating progenitors and stem cells have been reported to contribute to angiogenesis and arterial repair after injury. In the present study, we investigated whether the arterial wall could host permanently residing progenitor cells under physiological context. Methods and Results— Using the Hoechst-based flow cytometry method, we identified and isolated progenitor cells termed side population (SP) cells at a prevalence of 6.0±0.8% in the tunica media of adult mice aortas. Arterial SP cells expressed the ATP-binding cassette transporter subfamily G member 2, frequently present on SP cell surface, and displayed a Sca-1 + c-kit −/low Lin − CD34 −/low profile. They did not form myeloid or lymphoid hematopoietic colonies after plating in methylcellulose-based medium. Importantly, cultured SP cells were able to acquire the phenotype of endothelial cells (CD31, VE-cadherin, and von Willebrand factor expression) or of smooth muscle cells (α-smooth muscle actin, calponin, and smooth muscle myosin heavy chain expression), in presence of either vascular endothelial growth factor or transforming growth factor (TGF)-β1/PDGF-BB, respectively. Moreover, they generated vascular-like branching structures, composed of both VE-cadherin + cells and α-smooth muscle actin + cells on Matrigel. Conclusions— In this study, we provide the first evidence to our knowledge that in the adult mice, the normal arterial wall harbors SP cells with vascular progenitor properties. |
| Databáze: | OpenAIRE |
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