Goat Milk Epithelial Cells Are Highly Permissive to CAEV Infection in Vitro
| Autor: | Claudie Fornazero, Jean François Mornex, Jian Du, Yahia Chebloune, François Guiguen, Delphine Grezel, Sabine Balleydier, Jitka Durand, Laila Mselli-Lakhal, Colette Favier |
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| Přispěvatelé: | Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) |
| Rok vydání: | 1999 |
| Předmět: |
Arthritis-Encephalitis Virus
Caprine 040301 veterinary sciences [SDV]Life Sciences [q-bio] Cell Biology Virus 0403 veterinary science 03 medical and health sciences Virology medicine Animals Cells Cultured 030304 developmental biology Cytopathic effect 0303 health sciences Cell fusion Goats virus diseases Epithelial Cells 04 agricultural and veterinary sciences In vitro 3. Good health Titer medicine.anatomical_structure Milk Lentivirus Infections Colostrum Female Synovial membrane |
| Zdroj: | Virology Virology, Elsevier, 1999, 259 (1), pp.67-73. ⟨10.1006/viro.1999.9752⟩ |
| ISSN: | 0042-6822 1096-0341 |
| DOI: | 10.1006/viro.1999.9752 |
| Popis: | The main route of small ruminant lentivirus dissemination is the ingestion of infected cells present in colostrum and milk from infected animals. However, whether only macrophages or other cell subtypes are involved in this transmission is unknown. We derived epithelial cell cultures, 100% cytokeratin positive, from milk of naturally infected and noninfected goats. One such culture, derived from a naturally infected goat, constitutively produced a high titer of virus in the absence of any cytopathic effect. The other cultures, negative for natural lentivirus infection, were tested for their susceptibility to infection with the CAEV-CO strain and a French field isolate CAEV-3112. We showed that milk epithelial cells are easily infected by either virus and produce viruses at titers as high as those obtained in permissive goat synovial membrane cells. The CAEV-CO strain replicated in milk epithelial cells in absence of any cytopathic effect, whereas the CAEV-3112 field isolate induced both cell fusion and cell lysis. Our results suggest that CAEV-infected milk epithelial cells of small ruminants may play an important role in virus transmission and pathogenesis. |
| Databáze: | OpenAIRE |
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