Novel Wx alleles generated by base editing for improvement of rice grain quality
| Autor: | Hui Zhang, Fating Mei, Xiaomu Niu, Fei Su, Jinshan Zhang, Sheng Huang, Xiaorui Huang, Changle Ma, Xiaoguo Zhu, Jian-Kang Zhu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Plant Science Biology 01 natural sciences Biochemistry General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound Starch Synthase Amylose Nucleotide Allele Gene Alleles Plant Proteins Genetics chemistry.chemical_classification Gene Editing Oryza sativa Wild type food and beverages Rice grain Oryza 030104 developmental biology chemistry Edible Grain Cytosine 010606 plant biology & botany |
| Zdroj: | Journal of integrative plant biologyREFERENCES. 63(9) |
| ISSN: | 1744-7909 |
| Popis: | Amylose content (AC), which is regulated by the Waxy (Wx) gene, is a major indicator of eating and cooking quality (ECQ) in rice (Oryza sativa). Thus far, only a limited number of mutations in the N-terminal domain of Wx were found to have a major impact on the AC of rice grains and no mutations with such effects were reported for other regions of the Wx protein. Here, nucleotide substitutions in the middle region of Wx were generated by adenine and cytosine base editors. The nucleotide substitutions led to changes in 15 amino acid residues of Wx, and a series of novel Wx alleles with ACs of 0.3%-29.43% (wild type with AC of 19.87%) were obtained. Importantly, the waxyabe2 allele showed a "soft rice" AC, improved ECQ, favorable appearance, and no undesirable agronomic traits. The transgenes were removed from the waxyabe2 progeny, generating a promising breeding material for improving rice grain quality. |
| Databáze: | OpenAIRE |
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