Assessment of denitrifying bacterial composition in activated sludge
| Autor: | Bhavita Patel, Anuradha S. Nerurkar, Mrinal Shah, C. S. Srinandan |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Environmental Engineering
Bacteria Base Sequence Sewage biology Renewable Energy Sustainability and the Environment Pseudomonas Bioengineering Pseudomonas fluorescens Biodiversity General Medicine biology.organism_classification Polymerase Chain Reaction Microbiology Pseudomonas stutzeri Denitrifying bacteria Activated sludge Denitrification Brevundimonas diminuta Waste Management and Disposal Polymorphism Restriction Fragment Length Betaproteobacteria Pseudomonas mendocina DNA Primers |
| Zdroj: | Bioresource Technology. 102:9481-9489 |
| ISSN: | 0960-8524 |
| DOI: | 10.1016/j.biortech.2011.07.094 |
| Popis: | The abundance and structure of denitrifying bacterial community in different activated sludge samples were assessed, where the abundance of denitrifying functional genes showed nirS in the range of 10(4)-10(5), nosZ with 10(4)-10(6) and 16S rRNA gene in the range 10(9)-10(10) copy number per ml of sludge. The culturable approach revealed Pseudomonas sp. and Alcaligenes sp. to be numerically high, whereas culture independent method showed betaproteobacteria to dominate the sludge samples. Comamonas sp. and Pseudomonas fluorescens isolates showed efficient denitrification, while Pseudomonas mendocina, Pseudomonas stutzeri and Brevundimonas diminuta accumulated nitrite during denitrification. Numerically dominant RFLP OTUs of the nosZ gene from the fertilizer factory sludge samples clustered with the known isolates of betaproteobacteria. The data also suggests the presence of different truncated denitrifiers with high numbers in sludge habitat. |
| Databáze: | OpenAIRE |
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