Complete genomic sequence of Epstein-Barr virus in nasopharyngeal carcinoma cell line C666-1
| Autor: | Sau Dan Lee, Kevin Y. Yip, Ka Fai To, Ken Kai-Yuen Tso, Grace Tin-Yun Chung, Cathy Ka-Yan Mak, Siu Tim Cheung, Kwok Wai Lo |
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| Rok vydání: | 2013 |
| Předmět: |
Cancer Research
Epidemiology Short Report Biology medicine.disease_cause Genome Single-nucleotide variations symbols.namesake hemic and lymphatic diseases Consensus sequence medicine otorhinolaryngologic diseases Nasopharyngeal carcinoma Epstein-Barr virus Sanger sequencing Whole genome sequencing Genetics Massive parallel sequencing Phylogenetic analysis Whole-genome deep sequencing medicine.disease Epstein–Barr virus Virology Indels stomatognathic diseases Infectious Diseases Oncology GenBank symbols BNRF |
| Zdroj: | Infectious Agents and Cancer |
| ISSN: | 1750-9378 |
| Popis: | Background Nasopharyngeal carcinoma is a distinct type of head and neck cancer which is consistently associated with Epstein-Barr virus (EBV). The C666-1 cell line is the only in vitro native EBV-infected NPC cell model commonly used for study of the viral-host interaction. Nevertheless, the complete EBV genome sequence in this in vitro EBV-infected NPC model has not been characterized. Objective To determine the complete EBV genome sequence in C666-1 cells. Methods The C666-1 genome was sequenced by 100-bases pair-end massive parallel sequencing. Bioinformatics analysis was performed to extract the EBV sequences and construct an EBV consensus sequence map. PCR amplification and Sanger DNA sequencing were used for sequence validation and gap filling. A phylogenetic analysis of EBV strain in C666-1 cells and other reported EBV strains was performed. Results A 171,317 bp complete EBV genome of C666-1 was successfully constructed (GenBank accession number: KC617875). Phylogenetic analysis of EBV genome in C666-1 revealed that the C666-1 EBV strain is closely related to the reported strains in NPC primary tumors. Conclusion C666-1 contains a representative NPC-associated EBV genome and might serve as an important model for studying the roles or function of viral proteins in NPC tumorigenesis. |
| Databáze: | OpenAIRE |
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