Trends of beta-lactam antibiotic susceptibility in blood-borne methicillin-resistant Staphylococcus aureus (MRSA) and their linkage to the staphylococcal cassette chromosome mec (SCCmec) type
| Autor: | Kazunari Barada, Hideaki Hanaki, Shinsuke Ikeda, Keisuke Sunakawa, Hiroyuki Akama, Taiji Nakae, Yoshio Yamaguchi, Takashi Inamatsu |
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| Rok vydání: | 2006 |
| Předmět: |
Microbiology (medical)
Imipenem Staphylococcus aureus medicine.drug_class Antibiotics Cefazolin Bacteremia Biology medicine.disease_cause Microbiology Cloxacillin Antibiotic resistance Japan Drug Resistance Multiple Bacterial medicine Humans Pharmacology (medical) Retrospective Studies SCCmec biochemical phenomena metabolism and nutrition Chromosomes Bacterial Staphylococcal Infections bacterial infections and mycoses Methicillin-resistant Staphylococcus aureus Infectious Diseases Methicillin Resistance medicine.drug |
| Zdroj: | Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy. 13(4) |
| ISSN: | 1341-321X |
| Popis: | We investigated trends of beta-lactam antibiotic susceptibility in a total of 218 strains of blood-borne methicillin-resistant Staphylococcus aureus (MRSA) isolated from 1978 through 2002 at a middle-size geriatric hospital in Tokyo; the strains were classified by the MRSA marker, staphylococcal cassette chromosome mec (SCCmec). The minimum growth inhibitory concentration (MIC) of cloxacillin at which 50% of the strains were inhibited (MIC50) was 2 microg/ml in the strains isolated in 1978-1984 and 32 to 64 microg/ml in the strains isolated subsequently. Similarly, the MIC50 values of cefazolin and imipenem in the 1978-1984 isolates were 16 andor=0.25 microg/ml, respectively, and those in the strains isolated subsequently were 128 and 16 to 32 microg/ml, respectively. These results indicated that the MRSA strains isolated after 1985 had gained high-level resistance to beta-lactam antibiotics. The type of SCCmec was investigated by polymerase chain reaction (PCR). We observed an elevated frequency of type II SCCmec, from about 15% in the strains isolated in 1978-1984 to about 50% in the 1985-1989 isolates, and the frequency reached and remained at about 90% subsequently. These results imply that the high-level beta-lactam antibiotic resistance of the MRSA strains isolated after 1985 is most likely due to the increased integration of type II SCCmec. |
| Databáze: | OpenAIRE |
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