Align or not to align? Resolving species complexes within theCaloplaca saxicolagroup as a case study
| Autor: | Xavier Llimona, François Lutzoni, Benjamin D. Redelings, Miquel De Cáceres, Pere Navarro-Rosinés, Ester Gaya |
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| Rok vydání: | 2011 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Molecular Sequence Data Zoology DNA Ribosomal 010603 evolutionary biology 01 natural sciences Caloplaca 03 medical and health sciences Monophyly Ascomycota Genus Genetics Saxicola DNA Fungal Molecular Biology Phylogeny Ecology Evolution Behavior and Systematics biology Phylogenetic tree Bayes Theorem Cell Biology General Medicine 030108 mycology & parasitology biology.organism_classification Maximum parsimony Caloplaca saxicola Teloschistaceae |
| Zdroj: | Mycologia. 103:361-378 |
| ISSN: | 1557-2536 0027-5514 |
| Popis: | The Caloplaca saxicola group is the main group of saxicolous, lobed-effigurate species within genus Caloplaca (Teloschistaceae, lichen-forming Ascomycota). A recent monographic revision by the first author detected a wide range of morphological variation. To confront the phenotypically based circumscription of these taxa and to resolve their relationships morphological and ITS rDNA data were obtained for 56 individuals representing eight Calo- placa species belonging to the C. saxicola group. We tested the monophyly of these eight morphospecies by performing maximum parsimony, maximum like- lihood and two different types of Bayesian analyses (with and without a priori alignments). Restricting phylogenetic analyses to unambiguously aligned portions of ITS was sufficient to resolve, with high bootstrap support, five of the eight previously recognized species within the C. saxicola group. However, phylogenetic resolution of all or most of the eight species currently included as two distinct subgroups within the C. saxicola group was possible only by combining morphological characters and signal from ambiguously aligned regions with the unambiguously aligned ITS sites or when the entire ITS1 and 2 regions were not aligned a priori and included as an integral component of a Bayesian analysis (BAli-Phy). The C. arnoldii subgroup includes C. arnoldii, comprising four subspecies, and the C. saxicola subgroup encompasses seven species. Con- trary to the C. saxicola subgroup, monophyly of taxa included within the C. arnoldii subgroup and their relationships could not be resolved with combined ITS and morphological data. Unequivocal morpho- logical synapomorphies for all species except C. arnoldii and C. pusilla are recognized and presented. |
| Databáze: | OpenAIRE |
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