In vitro Anticancer Effects of
| Autor: | Vuanghao Lim, Emmanuel Jairaj Moses, Kang Zi Khor, Julia Joseph, Mohd Yusmaidie Aziz, Nozlena Abdul Samad |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
silver nanoparticles
Silver Cell Survival Biophysics Pharmaceutical Science Metal Nanoparticles Bioengineering Antineoplastic Agents 02 engineering and technology Chemistry Techniques Synthetic 010402 general chemistry 01 natural sciences Silver nanoparticle Biomaterials chemistry.chemical_compound International Journal of Nanomedicine Drug Discovery Humans Vernonia amygdalina Viability assay Fragmentation (cell biology) Cytotoxicity Original Research Ethanol biology Chemistry Plant Extracts Organic Chemistry apoptosis Green Chemistry Technology General Medicine 021001 nanoscience & nanotechnology biology.organism_classification 0104 chemical sciences Plant Leaves MCF-7 Apoptosis MCF-7 Cells Solvents cytotoxicity 0210 nano-technology Corrigendum Vernonia Nuclear chemistry DNA Damage |
| Zdroj: | International Journal of Nanomedicine |
| ISSN: | 1178-2013 |
| Popis: | Julia Joseph,1 Kang Zi Khor,1 Emmanuel Jairaj Moses,2 Vuanghao Lim,1 Mohd Yusmaidie Aziz,1 Nozlena Abdul Samad1 1Integrative Medicine Cluster, Advanced Medical and Dental Institute (AMDI), Sains@BERTAM, Universiti Sains Malaysia, Kepala Batas, 13200, Pulau Pinang, Malaysia; 2Regenerative Medicine Cluster, Advanced Medical and Dental Institute (AMDI), Sains@BERTAM, Universiti Sains Malaysia, Kepala Batas, 13200, Pulau Pinang, MalaysiaCorrespondence: Nozlena Abdul SamadIntegrative Medicine Cluster, Advanced Medical and Dental Institute (AMDI), Sains@BERTAM, Universiti Sains Malaysia, Kepala Batas, 13200, Pulau Pinang, MalaysiaTel +604-5622051Fax +604 - 562 2349Email nozlena@usm.myPurpose: Vernonia amygdalina (VA) is a traditional African herbal medicine that has been reported to possess anticancer properties. However, the anticancer properties of VA silver nanoparticles have not been studied. The aim of the study was to examine and evaluate the anticancer activities of VA leaf extracts and VA silver nanoparticles on the human breast cancer cell line, MCF-7.Methods: VA leaves were extracted using sequential extraction assisted with ultrasound using three different solvents: ethanol, 50% ethanol, and deionized water. The silver nanoparticles were synthesised with VA aqueous extract.Results: The ethanol extract and VA silver nanoparticles inhibit MCF-7 cell proliferation with an average half-maximal inhibitory concentration (IC50) value of 67μg/mL and 6.11μg/mL, respectively, after 72 hours of treatment. The ethanol extract and VA silver nanoparticles also caused G1 phase cell cycle arrest, induced apoptosis and nuclear fragmentation in MCF-7 cells.Conclusion: VA ethanol extracts and VA silver nanoparticles decreased the cell viability in MCF-7 cells in a time and dose-dependent manner by inducing apoptosis and causing DNA damage. Further research is needed to elucidate the mechanism of action of VA leaf extracts and VA silver nanoparticles. This study is the first to report on the anticancer activity of VA silver nanoparticles in MCF-7 cells.Keywords: Vernonia amygdalina, MCF-7, apoptosis, cytotoxicity, silver nanoparticles |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|